BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.
Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.
Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.Show MeSH
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Mentions: In contrast to virgin females, recently mated Drosophila females will reject a courting male (Wolfner, 1997). We have previously shown that BMP signaling in SCs is required for full induction of this female postmating behavior (Leiblich et al., 2012). We hypothesized that SC-derived exosomes might be involved in this function. Remarkably, blocking exosome production, using SC-specific expression of either ALiX-RNAi or dominant-negative Rab11, reduced the ability of males to prevent female remating (Fig. 8 A). Importantly, and similar to our observations when BMP signaling is blocked in SCs (Leiblich et al., 2012), we did not see any significant effect on egg laying, fecundity, or mating behavior for SC>ALiX-RNAi males (mating times, but not egg laying or fecundity, were reduced for Rab11DN; Fig. 8, B–F). Therefore, exosome secretion from SCs appears to be required to fully induce long-term postmating reprogramming of female behavior.
Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.