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BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.

Corrigan L, Redhai S, Leiblich A, Fan SJ, Perera SM, Patel R, Gandy C, Wainwright SM, Morris JF, Hamdy F, Goberdhan DC, Wilson C - J. Cell Biol. (2014)

Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.

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SC exosomes interact with female epithelial cells. (A) Schematic of female reproductive tract shown in B. mp, posterior mating plug (autofluorescent); ut, uterus; sr, seminal receptacle; spt, spermathecae; ovi, oviduct. (B–D) actin>CD8-RFP–expressing (B and C) or wild-type (D) females mated to SC>CD63-GFP males. (B) CD63-GFP exosomes are found in the uterus (green arrow) and oviduct (green asterisk). (C and D) These exosomes accumulate at the apical surface of female reproductive tract epithelial cells (arrows), which are either marked with CD8-RFP (C; Video 4) or unmarked (D). DAPI stains nuclei. In D, the reproductive tract is stained with an antibody against human CD63 to confirm that GFP and CD63 colocalize. Specific spermathecal cells exhibit weak autofluorescence. Bars: (B) 200 µm; (C) 20 µm; (D) 5 µm.
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fig7: SC exosomes interact with female epithelial cells. (A) Schematic of female reproductive tract shown in B. mp, posterior mating plug (autofluorescent); ut, uterus; sr, seminal receptacle; spt, spermathecae; ovi, oviduct. (B–D) actin>CD8-RFP–expressing (B and C) or wild-type (D) females mated to SC>CD63-GFP males. (B) CD63-GFP exosomes are found in the uterus (green arrow) and oviduct (green asterisk). (C and D) These exosomes accumulate at the apical surface of female reproductive tract epithelial cells (arrows), which are either marked with CD8-RFP (C; Video 4) or unmarked (D). DAPI stains nuclei. In D, the reproductive tract is stained with an antibody against human CD63 to confirm that GFP and CD63 colocalize. Specific spermathecal cells exhibit weak autofluorescence. Bars: (B) 200 µm; (C) 20 µm; (D) 5 µm.

Mentions: Interestingly, we noted accumulation of CD63-GFP exosomes at the surface of the reproductive tract either in the uterus or oviduct in 40% of females (Fig. 7; n = 34), suggesting that SC exosomes also interact with female epithelial cells. In some cases, fluorescent aggregates made contact with the epithelium (Fig. 7 C and Video 4), whereas in others, dispersed puncta aligned along the epithelial surface (Fig. 7 D). These interactions may reflect docking to female cell targets, either before internalization via endocytosis (Morelli et al., 2004) or possibly as part of a cell surface signaling mechanism. In summary, SC-derived exosomes do not seem to fuse to the AG epithelium in virgin males but do fuse to sperm and interact with female epithelial cells after mating.


BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.

Corrigan L, Redhai S, Leiblich A, Fan SJ, Perera SM, Patel R, Gandy C, Wainwright SM, Morris JF, Hamdy F, Goberdhan DC, Wilson C - J. Cell Biol. (2014)

SC exosomes interact with female epithelial cells. (A) Schematic of female reproductive tract shown in B. mp, posterior mating plug (autofluorescent); ut, uterus; sr, seminal receptacle; spt, spermathecae; ovi, oviduct. (B–D) actin>CD8-RFP–expressing (B and C) or wild-type (D) females mated to SC>CD63-GFP males. (B) CD63-GFP exosomes are found in the uterus (green arrow) and oviduct (green asterisk). (C and D) These exosomes accumulate at the apical surface of female reproductive tract epithelial cells (arrows), which are either marked with CD8-RFP (C; Video 4) or unmarked (D). DAPI stains nuclei. In D, the reproductive tract is stained with an antibody against human CD63 to confirm that GFP and CD63 colocalize. Specific spermathecal cells exhibit weak autofluorescence. Bars: (B) 200 µm; (C) 20 µm; (D) 5 µm.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4151142&req=5

fig7: SC exosomes interact with female epithelial cells. (A) Schematic of female reproductive tract shown in B. mp, posterior mating plug (autofluorescent); ut, uterus; sr, seminal receptacle; spt, spermathecae; ovi, oviduct. (B–D) actin>CD8-RFP–expressing (B and C) or wild-type (D) females mated to SC>CD63-GFP males. (B) CD63-GFP exosomes are found in the uterus (green arrow) and oviduct (green asterisk). (C and D) These exosomes accumulate at the apical surface of female reproductive tract epithelial cells (arrows), which are either marked with CD8-RFP (C; Video 4) or unmarked (D). DAPI stains nuclei. In D, the reproductive tract is stained with an antibody against human CD63 to confirm that GFP and CD63 colocalize. Specific spermathecal cells exhibit weak autofluorescence. Bars: (B) 200 µm; (C) 20 µm; (D) 5 µm.
Mentions: Interestingly, we noted accumulation of CD63-GFP exosomes at the surface of the reproductive tract either in the uterus or oviduct in 40% of females (Fig. 7; n = 34), suggesting that SC exosomes also interact with female epithelial cells. In some cases, fluorescent aggregates made contact with the epithelium (Fig. 7 C and Video 4), whereas in others, dispersed puncta aligned along the epithelial surface (Fig. 7 D). These interactions may reflect docking to female cell targets, either before internalization via endocytosis (Morelli et al., 2004) or possibly as part of a cell surface signaling mechanism. In summary, SC-derived exosomes do not seem to fuse to the AG epithelium in virgin males but do fuse to sperm and interact with female epithelial cells after mating.

Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.

Show MeSH
Related in: MedlinePlus