BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.
Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.
Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.Show MeSH
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Mentions: We investigated whether the interaction between SC exosomes and target cells primarily takes place after mating. SC>CD63-GFP males transferred fluorescent puncta into the lumen of the female reproductive tract during mating (Fig. 6, A–D). Multiply mated males of 11 d and older can sporadically transfer SCs to females (Leiblich et al., 2012). Importantly, all mating experiments performed in our current study were with 4-d-old males; we never observed transfer of fluorescent SCs in >50 mated females that we imaged, indicating that SC transfer is not involved in the phenotypes observed.
Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.