Limits...
BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.

Corrigan L, Redhai S, Leiblich A, Fan SJ, Perera SM, Patel R, Gandy C, Wainwright SM, Morris JF, Hamdy F, Goberdhan DC, Wilson C - J. Cell Biol. (2014)

Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.

Show MeSH

Related in: MedlinePlus

Real-time imaging reveals dynamic endolysosomal trafficking events. (A and B) Time-lapse imaging of 3-d-old SC>CD63-GFP males stained with LysoTracker red. (A) An mMVBL containing ILVs (asterisks) fuses with a large nonacidic SV (arrowheads; Video 1). Approximate outline of SC is marked. (B) A CD63-GFP–marked protrusion invaginates from the mMVBL membrane (arrows; 0 s) and seems to form a CD63-GFP–positive punctum in the next frame. Later, a line of peripheral acidic microdomains is seen below (48.9 s; see also Video 2). Bars, 5 µm.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4151142&req=5

fig4: Real-time imaging reveals dynamic endolysosomal trafficking events. (A and B) Time-lapse imaging of 3-d-old SC>CD63-GFP males stained with LysoTracker red. (A) An mMVBL containing ILVs (asterisks) fuses with a large nonacidic SV (arrowheads; Video 1). Approximate outline of SC is marked. (B) A CD63-GFP–marked protrusion invaginates from the mMVBL membrane (arrows; 0 s) and seems to form a CD63-GFP–positive punctum in the next frame. Later, a line of peripheral acidic microdomains is seen below (48.9 s; see also Video 2). Bars, 5 µm.

Mentions: Strikingly, real-time confocal imaging of SCs revealed nonacidic SVs fusing with mMVBLs and rapid exchange of limiting membrane components (Fig. 4 A and Video 1). Although the internal contents of these compartments did not mix freely, these findings suggest a surprising level of exchange between SVs and mMVBLs and might explain how CD63-GFP–positive structures accumulate in SVs (Fig. 1, E–H).


BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.

Corrigan L, Redhai S, Leiblich A, Fan SJ, Perera SM, Patel R, Gandy C, Wainwright SM, Morris JF, Hamdy F, Goberdhan DC, Wilson C - J. Cell Biol. (2014)

Real-time imaging reveals dynamic endolysosomal trafficking events. (A and B) Time-lapse imaging of 3-d-old SC>CD63-GFP males stained with LysoTracker red. (A) An mMVBL containing ILVs (asterisks) fuses with a large nonacidic SV (arrowheads; Video 1). Approximate outline of SC is marked. (B) A CD63-GFP–marked protrusion invaginates from the mMVBL membrane (arrows; 0 s) and seems to form a CD63-GFP–positive punctum in the next frame. Later, a line of peripheral acidic microdomains is seen below (48.9 s; see also Video 2). Bars, 5 µm.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4151142&req=5

fig4: Real-time imaging reveals dynamic endolysosomal trafficking events. (A and B) Time-lapse imaging of 3-d-old SC>CD63-GFP males stained with LysoTracker red. (A) An mMVBL containing ILVs (asterisks) fuses with a large nonacidic SV (arrowheads; Video 1). Approximate outline of SC is marked. (B) A CD63-GFP–marked protrusion invaginates from the mMVBL membrane (arrows; 0 s) and seems to form a CD63-GFP–positive punctum in the next frame. Later, a line of peripheral acidic microdomains is seen below (48.9 s; see also Video 2). Bars, 5 µm.
Mentions: Strikingly, real-time confocal imaging of SCs revealed nonacidic SVs fusing with mMVBLs and rapid exchange of limiting membrane components (Fig. 4 A and Video 1). Although the internal contents of these compartments did not mix freely, these findings suggest a surprising level of exchange between SVs and mMVBLs and might explain how CD63-GFP–positive structures accumulate in SVs (Fig. 1, E–H).

Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.

Show MeSH
Related in: MedlinePlus