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BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.

Corrigan L, Redhai S, Leiblich A, Fan SJ, Perera SM, Patel R, Gandy C, Wainwright SM, Morris JF, Hamdy F, Goberdhan DC, Wilson C - J. Cell Biol. (2014)

Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.

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Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.

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CD63-GFP traffics from secretory to endocytic compartments in SCs. (A–G) An 8-h pulse (at 28.5°C) of CD63-GFP expression was chased at 18°C for 0–60 h in virgin males, and proportions of cells with one or more LysoTracker red–positive iLEs (arrows in A, C, and E in apical sections; GFP positive in C and E) and mMVBLs (asterisks in B, D, and F in more basal views) that were CD63-GFP–positive were scored (G). Data shown are from a single representative experiment out of two repeats. The images in A–F are shown again in Fig. S3 alongside the corresponding single color channel images. Approximate outline of SC is marked in all images. **, P < 0.01; ***, P < 0.001; n > 24, pairwise comparisons, Fisher’s exact test. Bars, 5 µm.
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fig3: CD63-GFP traffics from secretory to endocytic compartments in SCs. (A–G) An 8-h pulse (at 28.5°C) of CD63-GFP expression was chased at 18°C for 0–60 h in virgin males, and proportions of cells with one or more LysoTracker red–positive iLEs (arrows in A, C, and E in apical sections; GFP positive in C and E) and mMVBLs (asterisks in B, D, and F in more basal views) that were CD63-GFP–positive were scored (G). Data shown are from a single representative experiment out of two repeats. The images in A–F are shown again in Fig. S3 alongside the corresponding single color channel images. Approximate outline of SC is marked in all images. **, P < 0.01; ***, P < 0.001; n > 24, pairwise comparisons, Fisher’s exact test. Bars, 5 µm.

Mentions: Rab GTPase signatures define different SC subcellular compartments. (A–D) SCs from 3-d-old males expressing different Rab-YFP constructs and stained with LysoTracker red. SCs have 5–10 small acidic Rab7-YFP–positive iLEs (A, arrowheads; most of these are usually located more apically than this confocal section; see Fig. 3) and 2.4 ± 0.9 (n = 11) Rab7-YFP–positive large vacuoles, 85% of which are acidic mMVBLs (A, asterisks; similar numbers [2.9 ± 0.5, n = 9] of large acidic compartments are seen in CD63-GFP–expressing SCs at this stage). Other Rab-YFP lines reveal Rab11-YFP–positive, nonacidic SVs (B, +; and D), many Rab5-YFP–positive small nonacidic compartments (C, arrowheads), and more rarely (4/16 SCs), a Rab5-YFP–positive small acidic compartment (C, arrows). In some fixed tub>Rab11-YFP SCs stained for Rab7, Rab7 colocalizes with Rab11-YFP in parts of the limiting membrane (D, arrow) of a single SV (D, #). DAPI stains nuclei (blue). Approximate outline of SC is marked in all images. Bars, 5 µm.


BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.

Corrigan L, Redhai S, Leiblich A, Fan SJ, Perera SM, Patel R, Gandy C, Wainwright SM, Morris JF, Hamdy F, Goberdhan DC, Wilson C - J. Cell Biol. (2014)

CD63-GFP traffics from secretory to endocytic compartments in SCs. (A–G) An 8-h pulse (at 28.5°C) of CD63-GFP expression was chased at 18°C for 0–60 h in virgin males, and proportions of cells with one or more LysoTracker red–positive iLEs (arrows in A, C, and E in apical sections; GFP positive in C and E) and mMVBLs (asterisks in B, D, and F in more basal views) that were CD63-GFP–positive were scored (G). Data shown are from a single representative experiment out of two repeats. The images in A–F are shown again in Fig. S3 alongside the corresponding single color channel images. Approximate outline of SC is marked in all images. **, P < 0.01; ***, P < 0.001; n > 24, pairwise comparisons, Fisher’s exact test. Bars, 5 µm.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4151142&req=5

fig3: CD63-GFP traffics from secretory to endocytic compartments in SCs. (A–G) An 8-h pulse (at 28.5°C) of CD63-GFP expression was chased at 18°C for 0–60 h in virgin males, and proportions of cells with one or more LysoTracker red–positive iLEs (arrows in A, C, and E in apical sections; GFP positive in C and E) and mMVBLs (asterisks in B, D, and F in more basal views) that were CD63-GFP–positive were scored (G). Data shown are from a single representative experiment out of two repeats. The images in A–F are shown again in Fig. S3 alongside the corresponding single color channel images. Approximate outline of SC is marked in all images. **, P < 0.01; ***, P < 0.001; n > 24, pairwise comparisons, Fisher’s exact test. Bars, 5 µm.
Mentions: Rab GTPase signatures define different SC subcellular compartments. (A–D) SCs from 3-d-old males expressing different Rab-YFP constructs and stained with LysoTracker red. SCs have 5–10 small acidic Rab7-YFP–positive iLEs (A, arrowheads; most of these are usually located more apically than this confocal section; see Fig. 3) and 2.4 ± 0.9 (n = 11) Rab7-YFP–positive large vacuoles, 85% of which are acidic mMVBLs (A, asterisks; similar numbers [2.9 ± 0.5, n = 9] of large acidic compartments are seen in CD63-GFP–expressing SCs at this stage). Other Rab-YFP lines reveal Rab11-YFP–positive, nonacidic SVs (B, +; and D), many Rab5-YFP–positive small nonacidic compartments (C, arrowheads), and more rarely (4/16 SCs), a Rab5-YFP–positive small acidic compartment (C, arrows). In some fixed tub>Rab11-YFP SCs stained for Rab7, Rab7 colocalizes with Rab11-YFP in parts of the limiting membrane (D, arrow) of a single SV (D, #). DAPI stains nuclei (blue). Approximate outline of SC is marked in all images. Bars, 5 µm.

Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.

Show MeSH
Related in: MedlinePlus