BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.
Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.
Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.Show MeSH
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Mentions: We analyzed the large SC compartments in more detail using LysoTracker red–stained living glands. Counting vacuoles by scanning through multiple focal planes revealed remarkably consistent numbers of large (>2 µm) and small (<2 µm) CD63-positive acidic compartments and large nonacidic CD63-positive compartments in each SC of 6-d-old adults (Fig. 1 I). Both classes of acidic compartments were also visible in non–CD63-GFP–expressing SCs (Figs. S1, G versus H; and 2, A–C) and therefore were not induced by CD63-GFP expression. We hypothesized that the smaller apical acidic compartments were immature late endosomes (iLEs; CD63-GFP+/LysoTracker+ and <2 µm; Fig. 1 I), and the large acidic compartments, at least one of which contained small CD63-GFP–positive puncta, were unusually large (≤10 µm in 6-d-old SCs) maturing MVBs or lysosomes (mMVBLs; CD63-GFP+/LysoTracker+ and >2 µm; Fig. 1 I). In addition, some, but not all, nonacidic SV compartments (CD63-GFP+/Lysotracker− and >2 µm; Fig. 1 I) contained larger CD63-GFP–positive intraluminal structures.
Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.