BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.
Bottom Line: Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success.Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling.These results demonstrate a new function for the MVB-exosome pathway in the reproductive tract that appears to be conserved across evolution.
Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.Show MeSH
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Mentions: Each AG secretes into a large lumen, which opens at its proximal end into the ejaculatory duct (Fig. 1, A–C). We expressed a GFP-tagged tetraspanin, CD63, a mammalian transmembrane exosome marker used previously to mark fly exosomes (Panáková et al., 2005; Gross, et al., 2012), independently in MCs and SCs (Figs. 1, A–C; and S1, A and B) using the GAL4/upstream activating sequence (UAS) system (Brand and Perrimon, 1993). In SCs of 3-d-old males, CD63-GFP localized to the limiting membrane of all large (>2-µm diameter) vacuoles and, in some cells, to the apical plasma membrane (Fig. 1 F). Not all vacuoles were identical, however; the majority appeared to be compartments previously described as secretory vacuoles (SVs; Rylett et al., 2007) because they had a dense core containing the secreted protease angiotensin-converting enzyme I (ANCE; Fig. 1, E and F). CD63-GFP–positive puncta, presumably representing intraluminal vesicles (ILVs), were also observed inside many vacuoles, especially in live specimens. These puncta were typically most prominent in at least one acidic compartment, stained with the vital acid-sensitive dye LysoTracker red (Fig. 1, G and H).
Affiliation: Department of Physiology, Anatomy and Genetics and Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX1 3QX, England, UK.