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Development and characterization of xenograft model systems for adenoid cystic carcinoma.

Moskaluk CA, Baras AS, Mancuso SA, Fan H, Davidson RJ, Dirks DC, Golden WL, Frierson HF - Lab. Invest. (2011)

Bottom Line: Adenoid cystic carcinoma (ACC) is one of the most common malignancies to arise in human salivary glands, and it also arises in the glandular tissue of other organ systems.As ACC is known to frequently contain a t(6;9) translocation that fuses the MYB and NFIB genes, fluorescence in situ hybridization (FISH) of 12 ACC xenograft models was performed that assayed MYB locus break-apart and MYB-NFIB locus fusion.The two related xenograft models (derived from primary and metastatic tumors, respectively, of the same human subject) were karyotyped, showing a t(1;6) translocation, suggesting MYB translocation to a novel fusion partner gene.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA. cam5p@virginia.edu

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Related in: MedlinePlus

Representative karyotypes of ACCX2 and ACCX6The karyotype of ACCX2 is shown with arrows demonstrating the translocation between chromosomes 1 and 6, an inverted 5 and loss of the Y chromosome. A representative karyotype of ACCX6 demonstrates the identical abnormalities. The karyotype designation for both ACCX2 and ACCX6 is as follows: 45, X, −Y, t(1;6)(p22;q13), inv(5)(q13q33).
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Figure 5: Representative karyotypes of ACCX2 and ACCX6The karyotype of ACCX2 is shown with arrows demonstrating the translocation between chromosomes 1 and 6, an inverted 5 and loss of the Y chromosome. A representative karyotype of ACCX6 demonstrates the identical abnormalities. The karyotype designation for both ACCX2 and ACCX6 is as follows: 45, X, −Y, t(1;6)(p22;q13), inv(5)(q13q33).

Mentions: Given that these related xenograft models appeared to have the MYB gene locus rearranged, but not fused to the characteristic NFIB locus on chromosome 9, karyotype analysis was performed to study their chromosomal structure. A total of 15 metaphase cells were available for analysis from ACCX2. The consensus karyotype for this xenograft was 45, X,-Y, t(1;6)(p22;q13), inv(5)(q13q33). Only 5 metaphases were found from ACCX6; however, all had the same karyotype as ACCX2: 45, X,-Y, t(1;6)(p22;q13), inv (5) (q13q33). Representative karyotypes are shown in Figure 5.


Development and characterization of xenograft model systems for adenoid cystic carcinoma.

Moskaluk CA, Baras AS, Mancuso SA, Fan H, Davidson RJ, Dirks DC, Golden WL, Frierson HF - Lab. Invest. (2011)

Representative karyotypes of ACCX2 and ACCX6The karyotype of ACCX2 is shown with arrows demonstrating the translocation between chromosomes 1 and 6, an inverted 5 and loss of the Y chromosome. A representative karyotype of ACCX6 demonstrates the identical abnormalities. The karyotype designation for both ACCX2 and ACCX6 is as follows: 45, X, −Y, t(1;6)(p22;q13), inv(5)(q13q33).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151120&req=5

Figure 5: Representative karyotypes of ACCX2 and ACCX6The karyotype of ACCX2 is shown with arrows demonstrating the translocation between chromosomes 1 and 6, an inverted 5 and loss of the Y chromosome. A representative karyotype of ACCX6 demonstrates the identical abnormalities. The karyotype designation for both ACCX2 and ACCX6 is as follows: 45, X, −Y, t(1;6)(p22;q13), inv(5)(q13q33).
Mentions: Given that these related xenograft models appeared to have the MYB gene locus rearranged, but not fused to the characteristic NFIB locus on chromosome 9, karyotype analysis was performed to study their chromosomal structure. A total of 15 metaphase cells were available for analysis from ACCX2. The consensus karyotype for this xenograft was 45, X,-Y, t(1;6)(p22;q13), inv(5)(q13q33). Only 5 metaphases were found from ACCX6; however, all had the same karyotype as ACCX2: 45, X,-Y, t(1;6)(p22;q13), inv (5) (q13q33). Representative karyotypes are shown in Figure 5.

Bottom Line: Adenoid cystic carcinoma (ACC) is one of the most common malignancies to arise in human salivary glands, and it also arises in the glandular tissue of other organ systems.As ACC is known to frequently contain a t(6;9) translocation that fuses the MYB and NFIB genes, fluorescence in situ hybridization (FISH) of 12 ACC xenograft models was performed that assayed MYB locus break-apart and MYB-NFIB locus fusion.The two related xenograft models (derived from primary and metastatic tumors, respectively, of the same human subject) were karyotyped, showing a t(1;6) translocation, suggesting MYB translocation to a novel fusion partner gene.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA. cam5p@virginia.edu

Show MeSH
Related in: MedlinePlus