Development and characterization of xenograft model systems for adenoid cystic carcinoma.
Bottom Line: Adenoid cystic carcinoma (ACC) is one of the most common malignancies to arise in human salivary glands, and it also arises in the glandular tissue of other organ systems.As ACC is known to frequently contain a t(6;9) translocation that fuses the MYB and NFIB genes, fluorescence in situ hybridization (FISH) of 12 ACC xenograft models was performed that assayed MYB locus break-apart and MYB-NFIB locus fusion.The two related xenograft models (derived from primary and metastatic tumors, respectively, of the same human subject) were karyotyped, showing a t(1;6) translocation, suggesting MYB translocation to a novel fusion partner gene.
Affiliation: Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA. email@example.comShow MeSH
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Mentions: Given that these related xenograft models appeared to have the MYB gene locus rearranged, but not fused to the characteristic NFIB locus on chromosome 9, karyotype analysis was performed to study their chromosomal structure. A total of 15 metaphase cells were available for analysis from ACCX2. The consensus karyotype for this xenograft was 45, X,-Y, t(1;6)(p22;q13), inv(5)(q13q33). Only 5 metaphases were found from ACCX6; however, all had the same karyotype as ACCX2: 45, X,-Y, t(1;6)(p22;q13), inv (5) (q13q33). Representative karyotypes are shown in Figure 5.
Affiliation: Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA. firstname.lastname@example.org