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Development and characterization of xenograft model systems for adenoid cystic carcinoma.

Moskaluk CA, Baras AS, Mancuso SA, Fan H, Davidson RJ, Dirks DC, Golden WL, Frierson HF - Lab. Invest. (2011)

Bottom Line: Adenoid cystic carcinoma (ACC) is one of the most common malignancies to arise in human salivary glands, and it also arises in the glandular tissue of other organ systems.As ACC is known to frequently contain a t(6;9) translocation that fuses the MYB and NFIB genes, fluorescence in situ hybridization (FISH) of 12 ACC xenograft models was performed that assayed MYB locus break-apart and MYB-NFIB locus fusion.The two related xenograft models (derived from primary and metastatic tumors, respectively, of the same human subject) were karyotyped, showing a t(1;6) translocation, suggesting MYB translocation to a novel fusion partner gene.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA. cam5p@virginia.edu

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Related in: MedlinePlus

Examples of MYB break-apart FISHPanel A: The 38 kilobase (kb) MYB gene locus is shown schematically, oriented to the centromeric (cen) and telomeric (tel) aspect of chromosome 6. The characteristic location of the breakpoint involved in the t(6;9) translocation is shown by a black arrow. The location of the BAC clones used as FISH probes are shown above the locus, (drawn to scale) and colored to match the fluor used in the FISH assays. Panel B: ACCX22 displaying 2 red/green/yellow signals indicating 2 intact MYB loci. Panel C: ACCX14 displaying 1 red/green/yellow signal, 1 red signal and 1 green signal consistent with a MYB locus rearrangement. Panel D: ACCX16 displaying 1 red/green/yellow signal and 1 green signal consistent with a MYB locus rearrangement. Panel E: ACCX19 displaying 2 red/green/yellow signals and 1 green signal consistent with a rearrangement of one MYB locus and a duplication of the wild-type MYB locus.
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Figure 3: Examples of MYB break-apart FISHPanel A: The 38 kilobase (kb) MYB gene locus is shown schematically, oriented to the centromeric (cen) and telomeric (tel) aspect of chromosome 6. The characteristic location of the breakpoint involved in the t(6;9) translocation is shown by a black arrow. The location of the BAC clones used as FISH probes are shown above the locus, (drawn to scale) and colored to match the fluor used in the FISH assays. Panel B: ACCX22 displaying 2 red/green/yellow signals indicating 2 intact MYB loci. Panel C: ACCX14 displaying 1 red/green/yellow signal, 1 red signal and 1 green signal consistent with a MYB locus rearrangement. Panel D: ACCX16 displaying 1 red/green/yellow signal and 1 green signal consistent with a MYB locus rearrangement. Panel E: ACCX19 displaying 2 red/green/yellow signals and 1 green signal consistent with a rearrangement of one MYB locus and a duplication of the wild-type MYB locus.

Mentions: The twelve xenograft cases that were actively being passaged were examined for the integrity of the MYB locus using the centromeric BAC probe RP11-104D9, labeled green with fluorescein and the telomeric BAC probe RP11-170P19 labeled red with 5-ROX. 11 of 12 xenograft models (92%) showed separation of one of the red/green probe pairs, or specific loss of the telomeric probe, consistent with rearrangement of the MYB gene locus (Table 3). Figure 3 shows representative examples of MYB break-apart FISH assays.


Development and characterization of xenograft model systems for adenoid cystic carcinoma.

Moskaluk CA, Baras AS, Mancuso SA, Fan H, Davidson RJ, Dirks DC, Golden WL, Frierson HF - Lab. Invest. (2011)

Examples of MYB break-apart FISHPanel A: The 38 kilobase (kb) MYB gene locus is shown schematically, oriented to the centromeric (cen) and telomeric (tel) aspect of chromosome 6. The characteristic location of the breakpoint involved in the t(6;9) translocation is shown by a black arrow. The location of the BAC clones used as FISH probes are shown above the locus, (drawn to scale) and colored to match the fluor used in the FISH assays. Panel B: ACCX22 displaying 2 red/green/yellow signals indicating 2 intact MYB loci. Panel C: ACCX14 displaying 1 red/green/yellow signal, 1 red signal and 1 green signal consistent with a MYB locus rearrangement. Panel D: ACCX16 displaying 1 red/green/yellow signal and 1 green signal consistent with a MYB locus rearrangement. Panel E: ACCX19 displaying 2 red/green/yellow signals and 1 green signal consistent with a rearrangement of one MYB locus and a duplication of the wild-type MYB locus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151120&req=5

Figure 3: Examples of MYB break-apart FISHPanel A: The 38 kilobase (kb) MYB gene locus is shown schematically, oriented to the centromeric (cen) and telomeric (tel) aspect of chromosome 6. The characteristic location of the breakpoint involved in the t(6;9) translocation is shown by a black arrow. The location of the BAC clones used as FISH probes are shown above the locus, (drawn to scale) and colored to match the fluor used in the FISH assays. Panel B: ACCX22 displaying 2 red/green/yellow signals indicating 2 intact MYB loci. Panel C: ACCX14 displaying 1 red/green/yellow signal, 1 red signal and 1 green signal consistent with a MYB locus rearrangement. Panel D: ACCX16 displaying 1 red/green/yellow signal and 1 green signal consistent with a MYB locus rearrangement. Panel E: ACCX19 displaying 2 red/green/yellow signals and 1 green signal consistent with a rearrangement of one MYB locus and a duplication of the wild-type MYB locus.
Mentions: The twelve xenograft cases that were actively being passaged were examined for the integrity of the MYB locus using the centromeric BAC probe RP11-104D9, labeled green with fluorescein and the telomeric BAC probe RP11-170P19 labeled red with 5-ROX. 11 of 12 xenograft models (92%) showed separation of one of the red/green probe pairs, or specific loss of the telomeric probe, consistent with rearrangement of the MYB gene locus (Table 3). Figure 3 shows representative examples of MYB break-apart FISH assays.

Bottom Line: Adenoid cystic carcinoma (ACC) is one of the most common malignancies to arise in human salivary glands, and it also arises in the glandular tissue of other organ systems.As ACC is known to frequently contain a t(6;9) translocation that fuses the MYB and NFIB genes, fluorescence in situ hybridization (FISH) of 12 ACC xenograft models was performed that assayed MYB locus break-apart and MYB-NFIB locus fusion.The two related xenograft models (derived from primary and metastatic tumors, respectively, of the same human subject) were karyotyped, showing a t(1;6) translocation, suggesting MYB translocation to a novel fusion partner gene.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA. cam5p@virginia.edu

Show MeSH
Related in: MedlinePlus