Development and characterization of xenograft model systems for adenoid cystic carcinoma.
Bottom Line: Adenoid cystic carcinoma (ACC) is one of the most common malignancies to arise in human salivary glands, and it also arises in the glandular tissue of other organ systems.As ACC is known to frequently contain a t(6;9) translocation that fuses the MYB and NFIB genes, fluorescence in situ hybridization (FISH) of 12 ACC xenograft models was performed that assayed MYB locus break-apart and MYB-NFIB locus fusion.The two related xenograft models (derived from primary and metastatic tumors, respectively, of the same human subject) were karyotyped, showing a t(1;6) translocation, suggesting MYB translocation to a novel fusion partner gene.
Affiliation: Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA. email@example.comShow MeSH
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Mentions: The twelve xenograft cases that were actively being passaged were examined for the integrity of the MYB locus using the centromeric BAC probe RP11-104D9, labeled green with fluorescein and the telomeric BAC probe RP11-170P19 labeled red with 5-ROX. 11 of 12 xenograft models (92%) showed separation of one of the red/green probe pairs, or specific loss of the telomeric probe, consistent with rearrangement of the MYB gene locus (Table 3). Figure 3 shows representative examples of MYB break-apart FISH assays.
Affiliation: Department of Pathology, University of Virginia, Charlottesville, VA 22908, USA. firstname.lastname@example.org