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Fabrication of graphene-isolated-Au-nanocrystal nanostructures for multimodal cell imaging and photothermal-enhanced chemotherapy.

Bian X, Song ZL, Qian Y, Gao W, Cheng ZQ, Chen L, Liang H, Ding D, Nie XK, Chen Z, Tan W - Sci Rep (2014)

Bottom Line: First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes.Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy.The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

View Article: PubMed Central - PubMed

Affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082, China.

ABSTRACT
Using nanomaterials to develop multimodal systems has generated cutting-edge biomedical functions. Herein, we develop a simple chemical-vapor-deposition method to fabricate graphene-isolated-Au-nanocrystal (GIAN) nanostructures. A thin layer of graphene is precisely deposited on the surfaces of gold nanocrystals to enable unique capabilities. First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes. Second, GIANs can be used for multimodal cell imaging by both Raman scattering and near-infrared (NIR) two-photon luminescence. Third, GIANs provide a platform for loading anticancer drugs such as doxorubicin (DOX) for therapy. Finally, their NIR absorption properties give GIANs photothermal therapeutic capability in combination with chemotherapy. Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy. The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

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Photothermal enhanced chemotherapy with GIANs.(a) Schematic illustration of NIR photothermal enhanced chemotherapy mechanism of GIAN/DOX complexes. (b) UV-Vis characterization of the DOX-loaded GIANs. Inset: digital photo of the DOX, GIAN, and GIAN/DOX solutions. (c) Fluorescence spectroscopy characterization of the DOX loading efficiency. (d) Cell viability of MCF-7 cells with and without NIR laser irradiation after incubation with free DOX, GIAN, and GIAN/DOX, respectively.
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f6: Photothermal enhanced chemotherapy with GIANs.(a) Schematic illustration of NIR photothermal enhanced chemotherapy mechanism of GIAN/DOX complexes. (b) UV-Vis characterization of the DOX-loaded GIANs. Inset: digital photo of the DOX, GIAN, and GIAN/DOX solutions. (c) Fluorescence spectroscopy characterization of the DOX loading efficiency. (d) Cell viability of MCF-7 cells with and without NIR laser irradiation after incubation with free DOX, GIAN, and GIAN/DOX, respectively.

Mentions: Chemotherapy is another common therapy for cancer treatments. Combining chemotherapy with photothermal therapy would significantly enhance therapeutic efficiency. DOX is a widely used anthracycline antibiotic to treat various cancers56. GIAN proved to be a good substrate for loading DOX via π-π stacking (Fig. 6a). Moreover, GIAN/DOX complexes constitute a good platform for enhancing therapeutic efficiency through combining chemotherapy and hyperthermia. Fig. 6a is a schematic illustration of the NIR photothermal-enhanced chemotherapy mechanism. After GIAN/DOX endocytosing into the cancer cells, the DOX was partially released from the GIAN surface by decreasing the pH. As described below, the NIR photothermal effect of the GIAN could be further utilized to control the unloading of the chemotherapeutic DOX molecules.


Fabrication of graphene-isolated-Au-nanocrystal nanostructures for multimodal cell imaging and photothermal-enhanced chemotherapy.

Bian X, Song ZL, Qian Y, Gao W, Cheng ZQ, Chen L, Liang H, Ding D, Nie XK, Chen Z, Tan W - Sci Rep (2014)

Photothermal enhanced chemotherapy with GIANs.(a) Schematic illustration of NIR photothermal enhanced chemotherapy mechanism of GIAN/DOX complexes. (b) UV-Vis characterization of the DOX-loaded GIANs. Inset: digital photo of the DOX, GIAN, and GIAN/DOX solutions. (c) Fluorescence spectroscopy characterization of the DOX loading efficiency. (d) Cell viability of MCF-7 cells with and without NIR laser irradiation after incubation with free DOX, GIAN, and GIAN/DOX, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151100&req=5

f6: Photothermal enhanced chemotherapy with GIANs.(a) Schematic illustration of NIR photothermal enhanced chemotherapy mechanism of GIAN/DOX complexes. (b) UV-Vis characterization of the DOX-loaded GIANs. Inset: digital photo of the DOX, GIAN, and GIAN/DOX solutions. (c) Fluorescence spectroscopy characterization of the DOX loading efficiency. (d) Cell viability of MCF-7 cells with and without NIR laser irradiation after incubation with free DOX, GIAN, and GIAN/DOX, respectively.
Mentions: Chemotherapy is another common therapy for cancer treatments. Combining chemotherapy with photothermal therapy would significantly enhance therapeutic efficiency. DOX is a widely used anthracycline antibiotic to treat various cancers56. GIAN proved to be a good substrate for loading DOX via π-π stacking (Fig. 6a). Moreover, GIAN/DOX complexes constitute a good platform for enhancing therapeutic efficiency through combining chemotherapy and hyperthermia. Fig. 6a is a schematic illustration of the NIR photothermal-enhanced chemotherapy mechanism. After GIAN/DOX endocytosing into the cancer cells, the DOX was partially released from the GIAN surface by decreasing the pH. As described below, the NIR photothermal effect of the GIAN could be further utilized to control the unloading of the chemotherapeutic DOX molecules.

Bottom Line: First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes.Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy.The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

View Article: PubMed Central - PubMed

Affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082, China.

ABSTRACT
Using nanomaterials to develop multimodal systems has generated cutting-edge biomedical functions. Herein, we develop a simple chemical-vapor-deposition method to fabricate graphene-isolated-Au-nanocrystal (GIAN) nanostructures. A thin layer of graphene is precisely deposited on the surfaces of gold nanocrystals to enable unique capabilities. First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes. Second, GIANs can be used for multimodal cell imaging by both Raman scattering and near-infrared (NIR) two-photon luminescence. Third, GIANs provide a platform for loading anticancer drugs such as doxorubicin (DOX) for therapy. Finally, their NIR absorption properties give GIANs photothermal therapeutic capability in combination with chemotherapy. Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy. The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

Show MeSH