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Fabrication of graphene-isolated-Au-nanocrystal nanostructures for multimodal cell imaging and photothermal-enhanced chemotherapy.

Bian X, Song ZL, Qian Y, Gao W, Cheng ZQ, Chen L, Liang H, Ding D, Nie XK, Chen Z, Tan W - Sci Rep (2014)

Bottom Line: First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes.Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy.The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

View Article: PubMed Central - PubMed

Affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082, China.

ABSTRACT
Using nanomaterials to develop multimodal systems has generated cutting-edge biomedical functions. Herein, we develop a simple chemical-vapor-deposition method to fabricate graphene-isolated-Au-nanocrystal (GIAN) nanostructures. A thin layer of graphene is precisely deposited on the surfaces of gold nanocrystals to enable unique capabilities. First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes. Second, GIANs can be used for multimodal cell imaging by both Raman scattering and near-infrared (NIR) two-photon luminescence. Third, GIANs provide a platform for loading anticancer drugs such as doxorubicin (DOX) for therapy. Finally, their NIR absorption properties give GIANs photothermal therapeutic capability in combination with chemotherapy. Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy. The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

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Targeted cell imaging with GIANs.(a) Schematic illustration of Sgc8 aptamer-functionalized GIAN. (b) Gel electrophoresis characterization of aptamer-functionalized GIANs. (c) Fluorescence characterization of aptamer-functionalized GIANs. (d) TPL confocal images of HeLa and 95-C cells incubated with GIAN and GIAN-Sgc8. BF: bright field, scale bar: 10 μm.
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f4: Targeted cell imaging with GIANs.(a) Schematic illustration of Sgc8 aptamer-functionalized GIAN. (b) Gel electrophoresis characterization of aptamer-functionalized GIANs. (c) Fluorescence characterization of aptamer-functionalized GIANs. (d) TPL confocal images of HeLa and 95-C cells incubated with GIAN and GIAN-Sgc8. BF: bright field, scale bar: 10 μm.

Mentions: The GIAN graphene shell is an ideal platform for biomolecule functionalization. In particular, we functionalized GIANs with Sgc8 aptamers, which selectively bind to protein tyrosine kinase 7 (PTK7) cell membrane proteins, to investigate the targeted imaging capability of GIANs49. Single-stranded nucleic acids are known to adsorb on a graphene surface through strong π-π interactions, thereby providing a simple and efficient way to functionalize graphitic nanomaterials505152. Fig. 4a schematically illustrates Sgc8 aptamer-functionalized GIANs. We designed the Sgc8 aptamer into a hairpin structure with an eight-A-base tail, which helps the aptamer to anchor on the graphene shell, since the A base has strong binding affinity with the graphene surface53, while, as described below, aptamer activity is maintained.


Fabrication of graphene-isolated-Au-nanocrystal nanostructures for multimodal cell imaging and photothermal-enhanced chemotherapy.

Bian X, Song ZL, Qian Y, Gao W, Cheng ZQ, Chen L, Liang H, Ding D, Nie XK, Chen Z, Tan W - Sci Rep (2014)

Targeted cell imaging with GIANs.(a) Schematic illustration of Sgc8 aptamer-functionalized GIAN. (b) Gel electrophoresis characterization of aptamer-functionalized GIANs. (c) Fluorescence characterization of aptamer-functionalized GIANs. (d) TPL confocal images of HeLa and 95-C cells incubated with GIAN and GIAN-Sgc8. BF: bright field, scale bar: 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151100&req=5

f4: Targeted cell imaging with GIANs.(a) Schematic illustration of Sgc8 aptamer-functionalized GIAN. (b) Gel electrophoresis characterization of aptamer-functionalized GIANs. (c) Fluorescence characterization of aptamer-functionalized GIANs. (d) TPL confocal images of HeLa and 95-C cells incubated with GIAN and GIAN-Sgc8. BF: bright field, scale bar: 10 μm.
Mentions: The GIAN graphene shell is an ideal platform for biomolecule functionalization. In particular, we functionalized GIANs with Sgc8 aptamers, which selectively bind to protein tyrosine kinase 7 (PTK7) cell membrane proteins, to investigate the targeted imaging capability of GIANs49. Single-stranded nucleic acids are known to adsorb on a graphene surface through strong π-π interactions, thereby providing a simple and efficient way to functionalize graphitic nanomaterials505152. Fig. 4a schematically illustrates Sgc8 aptamer-functionalized GIANs. We designed the Sgc8 aptamer into a hairpin structure with an eight-A-base tail, which helps the aptamer to anchor on the graphene shell, since the A base has strong binding affinity with the graphene surface53, while, as described below, aptamer activity is maintained.

Bottom Line: First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes.Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy.The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

View Article: PubMed Central - PubMed

Affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082, China.

ABSTRACT
Using nanomaterials to develop multimodal systems has generated cutting-edge biomedical functions. Herein, we develop a simple chemical-vapor-deposition method to fabricate graphene-isolated-Au-nanocrystal (GIAN) nanostructures. A thin layer of graphene is precisely deposited on the surfaces of gold nanocrystals to enable unique capabilities. First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes. Second, GIANs can be used for multimodal cell imaging by both Raman scattering and near-infrared (NIR) two-photon luminescence. Third, GIANs provide a platform for loading anticancer drugs such as doxorubicin (DOX) for therapy. Finally, their NIR absorption properties give GIANs photothermal therapeutic capability in combination with chemotherapy. Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy. The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

Show MeSH