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Fabrication of graphene-isolated-Au-nanocrystal nanostructures for multimodal cell imaging and photothermal-enhanced chemotherapy.

Bian X, Song ZL, Qian Y, Gao W, Cheng ZQ, Chen L, Liang H, Ding D, Nie XK, Chen Z, Tan W - Sci Rep (2014)

Bottom Line: First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes.Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy.The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

View Article: PubMed Central - PubMed

Affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082, China.

ABSTRACT
Using nanomaterials to develop multimodal systems has generated cutting-edge biomedical functions. Herein, we develop a simple chemical-vapor-deposition method to fabricate graphene-isolated-Au-nanocrystal (GIAN) nanostructures. A thin layer of graphene is precisely deposited on the surfaces of gold nanocrystals to enable unique capabilities. First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes. Second, GIANs can be used for multimodal cell imaging by both Raman scattering and near-infrared (NIR) two-photon luminescence. Third, GIANs provide a platform for loading anticancer drugs such as doxorubicin (DOX) for therapy. Finally, their NIR absorption properties give GIANs photothermal therapeutic capability in combination with chemotherapy. Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy. The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

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TPL confocal images of MCF-7 cells without (a) and with (b) GIAN staining.Scale bar: 10 μm.
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f3: TPL confocal images of MCF-7 cells without (a) and with (b) GIAN staining.Scale bar: 10 μm.

Mentions: The TPL properties of GIANs were investigated for cell imaging. A wavelength of 850 nm was utilized as the excitation laser, while emission was collected with a two-photon microscope. As shown in Fig. 3, MCF-7 cancer cells incubated with GIANs demonstrated bright TPL signals. However, in the absence of GIAN incubation, no obvious TPL signal of control MCF-7 cells was observed (Fig. 3a), in agreement with the low NIR light absorption of biosamples. GIANs demonstrated good biocompatibility, and bright TPL signals were observed in the MCF-7 cells stained with GIANs (Fig. 3b). Consistent with the Raman images, the TPL signals were distributed throughout the cellular cytoplasm. Both the Au nanocrystal core and graphene outer layer of the GIANs are believed to contribute to the TPL signals (Supplementary, Fig. S4). The GIANs could exhibit TPL signals from the surface plasmon resonance of the Au nanocrystal core4445, as well as from the two-photon absorption of the sp3 hybridization state of the graphene shell464748.


Fabrication of graphene-isolated-Au-nanocrystal nanostructures for multimodal cell imaging and photothermal-enhanced chemotherapy.

Bian X, Song ZL, Qian Y, Gao W, Cheng ZQ, Chen L, Liang H, Ding D, Nie XK, Chen Z, Tan W - Sci Rep (2014)

TPL confocal images of MCF-7 cells without (a) and with (b) GIAN staining.Scale bar: 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151100&req=5

f3: TPL confocal images of MCF-7 cells without (a) and with (b) GIAN staining.Scale bar: 10 μm.
Mentions: The TPL properties of GIANs were investigated for cell imaging. A wavelength of 850 nm was utilized as the excitation laser, while emission was collected with a two-photon microscope. As shown in Fig. 3, MCF-7 cancer cells incubated with GIANs demonstrated bright TPL signals. However, in the absence of GIAN incubation, no obvious TPL signal of control MCF-7 cells was observed (Fig. 3a), in agreement with the low NIR light absorption of biosamples. GIANs demonstrated good biocompatibility, and bright TPL signals were observed in the MCF-7 cells stained with GIANs (Fig. 3b). Consistent with the Raman images, the TPL signals were distributed throughout the cellular cytoplasm. Both the Au nanocrystal core and graphene outer layer of the GIANs are believed to contribute to the TPL signals (Supplementary, Fig. S4). The GIANs could exhibit TPL signals from the surface plasmon resonance of the Au nanocrystal core4445, as well as from the two-photon absorption of the sp3 hybridization state of the graphene shell464748.

Bottom Line: First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes.Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy.The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

View Article: PubMed Central - PubMed

Affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082, China.

ABSTRACT
Using nanomaterials to develop multimodal systems has generated cutting-edge biomedical functions. Herein, we develop a simple chemical-vapor-deposition method to fabricate graphene-isolated-Au-nanocrystal (GIAN) nanostructures. A thin layer of graphene is precisely deposited on the surfaces of gold nanocrystals to enable unique capabilities. First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes. Second, GIANs can be used for multimodal cell imaging by both Raman scattering and near-infrared (NIR) two-photon luminescence. Third, GIANs provide a platform for loading anticancer drugs such as doxorubicin (DOX) for therapy. Finally, their NIR absorption properties give GIANs photothermal therapeutic capability in combination with chemotherapy. Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy. The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.

Show MeSH