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Microtubules provide directional information for core PCP function.

Matis M, Russler-Germain DA, Hu Q, Tomlin CJ, Axelrod JD - Elife (2014)

Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States matism@uni-muenster.de.

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Overexpression of Wnt4 affects MT polarity.(A) Wing hair and MT polarity in wildtype wing. (B) Wing hair and MT polarity in en-Gal4 UAS-Wnt4 are disturbed in wildtype cells outside the expressing area of Wnt4 between L3 and L4 veins. Note that wing hairs are pointing toward posterior region where Wnt4 is expressed and that MTs are oriented along the Wnt4 gradient generated outside the expression area of Wnt4. (C) Orientations of MTs in the wildtype cells between L3 and L4 veins calculated using OrientationJ.DOI:http://dx.doi.org/10.7554/eLife.02893.016
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fig3s7: Overexpression of Wnt4 affects MT polarity.(A) Wing hair and MT polarity in wildtype wing. (B) Wing hair and MT polarity in en-Gal4 UAS-Wnt4 are disturbed in wildtype cells outside the expressing area of Wnt4 between L3 and L4 veins. Note that wing hairs are pointing toward posterior region where Wnt4 is expressed and that MTs are oriented along the Wnt4 gradient generated outside the expression area of Wnt4. (C) Orientations of MTs in the wildtype cells between L3 and L4 veins calculated using OrientationJ.DOI:http://dx.doi.org/10.7554/eLife.02893.016

Mentions: Distal (peripheral) polarity is independent of Ft function; polarity in this region may depend on a spatial signal perhaps originating from the wing margin. A candidate for this signal is the proposed redundant functions of Wnt4 and Wg. Both are expressed at the wing margin, combined loss-of-function produces a mild polarity phenotype, and overexpression of Wnt4 to a much greater extent than Wg perturbs hair polarity (Lawrence et al., 2002; Lim et al., 2005; Wu et al., 2013). Based on a cell culture assay, these Wnts were suggested to impact core PCP function by blocking interactions between Fz and Vang. However, our observation that a Ft independent signal might polarize MTs near the wing margin suggests that other possible mechanisms should be considered. Notably Wnt4 (but not Wg) overexpression reorganizes MTs (Figure 3—figure supplement 7), suggesting that a different possible mechanism for Wnt4 function should be entertained.


Microtubules provide directional information for core PCP function.

Matis M, Russler-Germain DA, Hu Q, Tomlin CJ, Axelrod JD - Elife (2014)

Overexpression of Wnt4 affects MT polarity.(A) Wing hair and MT polarity in wildtype wing. (B) Wing hair and MT polarity in en-Gal4 UAS-Wnt4 are disturbed in wildtype cells outside the expressing area of Wnt4 between L3 and L4 veins. Note that wing hairs are pointing toward posterior region where Wnt4 is expressed and that MTs are oriented along the Wnt4 gradient generated outside the expression area of Wnt4. (C) Orientations of MTs in the wildtype cells between L3 and L4 veins calculated using OrientationJ.DOI:http://dx.doi.org/10.7554/eLife.02893.016
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151085&req=5

fig3s7: Overexpression of Wnt4 affects MT polarity.(A) Wing hair and MT polarity in wildtype wing. (B) Wing hair and MT polarity in en-Gal4 UAS-Wnt4 are disturbed in wildtype cells outside the expressing area of Wnt4 between L3 and L4 veins. Note that wing hairs are pointing toward posterior region where Wnt4 is expressed and that MTs are oriented along the Wnt4 gradient generated outside the expression area of Wnt4. (C) Orientations of MTs in the wildtype cells between L3 and L4 veins calculated using OrientationJ.DOI:http://dx.doi.org/10.7554/eLife.02893.016
Mentions: Distal (peripheral) polarity is independent of Ft function; polarity in this region may depend on a spatial signal perhaps originating from the wing margin. A candidate for this signal is the proposed redundant functions of Wnt4 and Wg. Both are expressed at the wing margin, combined loss-of-function produces a mild polarity phenotype, and overexpression of Wnt4 to a much greater extent than Wg perturbs hair polarity (Lawrence et al., 2002; Lim et al., 2005; Wu et al., 2013). Based on a cell culture assay, these Wnts were suggested to impact core PCP function by blocking interactions between Fz and Vang. However, our observation that a Ft independent signal might polarize MTs near the wing margin suggests that other possible mechanisms should be considered. Notably Wnt4 (but not Wg) overexpression reorganizes MTs (Figure 3—figure supplement 7), suggesting that a different possible mechanism for Wnt4 function should be entertained.

Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States matism@uni-muenster.de.

Show MeSH
Related in: MedlinePlus