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Microtubules provide directional information for core PCP function.

Matis M, Russler-Germain DA, Hu Q, Tomlin CJ, Axelrod JD - Elife (2014)

Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States matism@uni-muenster.de.

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Related in: MedlinePlus

Wing hair polarity in ft mutant wings reflects core PCP orientation in these wings.(A) Hair polarity in wildtype wing. (B) Hair polarity in ftl(2) fd/ftGRVActP-Gal4 UAS-FtΔECDΔN-1 adult wing (left top) and core PCP polarity in 28 hAPF pupal wing (middle) in same genotype. Right panel is high magnification image of middle panel box. (C) Hair polarity in ds38kfjN7/dsUA071fjd1; UAS-Ds/TubP-Gal4 adult wing (left top) and core PCP polarity in 28 hAPF pupal wing (middle) in same genotype. Right panel is high magnification image of middle panel box. Orientations of cell boundaries are color-coded using OrientationJ (18).DOI:http://dx.doi.org/10.7554/eLife.02893.015
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fig3s6: Wing hair polarity in ft mutant wings reflects core PCP orientation in these wings.(A) Hair polarity in wildtype wing. (B) Hair polarity in ftl(2) fd/ftGRVActP-Gal4 UAS-FtΔECDΔN-1 adult wing (left top) and core PCP polarity in 28 hAPF pupal wing (middle) in same genotype. Right panel is high magnification image of middle panel box. (C) Hair polarity in ds38kfjN7/dsUA071fjd1; UAS-Ds/TubP-Gal4 adult wing (left top) and core PCP polarity in 28 hAPF pupal wing (middle) in same genotype. Right panel is high magnification image of middle panel box. Orientations of cell boundaries are color-coded using OrientationJ (18).DOI:http://dx.doi.org/10.7554/eLife.02893.015


Microtubules provide directional information for core PCP function.

Matis M, Russler-Germain DA, Hu Q, Tomlin CJ, Axelrod JD - Elife (2014)

Wing hair polarity in ft mutant wings reflects core PCP orientation in these wings.(A) Hair polarity in wildtype wing. (B) Hair polarity in ftl(2) fd/ftGRVActP-Gal4 UAS-FtΔECDΔN-1 adult wing (left top) and core PCP polarity in 28 hAPF pupal wing (middle) in same genotype. Right panel is high magnification image of middle panel box. (C) Hair polarity in ds38kfjN7/dsUA071fjd1; UAS-Ds/TubP-Gal4 adult wing (left top) and core PCP polarity in 28 hAPF pupal wing (middle) in same genotype. Right panel is high magnification image of middle panel box. Orientations of cell boundaries are color-coded using OrientationJ (18).DOI:http://dx.doi.org/10.7554/eLife.02893.015
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151085&req=5

fig3s6: Wing hair polarity in ft mutant wings reflects core PCP orientation in these wings.(A) Hair polarity in wildtype wing. (B) Hair polarity in ftl(2) fd/ftGRVActP-Gal4 UAS-FtΔECDΔN-1 adult wing (left top) and core PCP polarity in 28 hAPF pupal wing (middle) in same genotype. Right panel is high magnification image of middle panel box. (C) Hair polarity in ds38kfjN7/dsUA071fjd1; UAS-Ds/TubP-Gal4 adult wing (left top) and core PCP polarity in 28 hAPF pupal wing (middle) in same genotype. Right panel is high magnification image of middle panel box. Orientations of cell boundaries are color-coded using OrientationJ (18).DOI:http://dx.doi.org/10.7554/eLife.02893.015
Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States matism@uni-muenster.de.

Show MeSH
Related in: MedlinePlus