Microtubules provide directional information for core PCP function.
Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.
Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States firstname.lastname@example.org.Show MeSH
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Mentions: One exception is the posterior margin of the wing, where Ds often appears to be oriented more posteriorly than is Fmi, though overall levels of asymmetry are modest (Figure 3—figure supplement 4, box 5). In this region, Ds and Fmi polarities therefore appear to be less tightly coupled. While we do not know the reason for this, we can speculatively suggest several possibilities. These include (1) the tendency for the core system to promote local alignment producing a more parallel arrangement than would a direct readout of the Ds pattern; (2) that oppositely oriented Ft-Ds heterodimers are unevenly distributed despite the even distribution of total Ds; (3) the tendency of MTs to align along the long axis of the cell may be stronger than the influence of Ft-Ds. Consistent with this, MT orientation correlates more strongly with the long axis of cells than with the Ds asymmetry (Figure 3—figure supplement 4, box 5). Finally, (4) we cannot rule out the possibility that other unknown signals may also be acting on MT orientation (see ‘An independent directional signal in the wing periphery?’).
Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States email@example.com.