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Microtubules provide directional information for core PCP function.

Matis M, Russler-Germain DA, Hu Q, Tomlin CJ, Axelrod JD - Elife (2014)

Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States matism@uni-muenster.de.

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Orientations of Ds gradient and core proteins correlate in late pupal wing (24 hAPF).(A and B) Images of 24 hAPF and 30 hAPF pupal wings showing Ds staining and Dsh::GFP. (C) Note the strong overlap of Fmi and Ds subcellular localization in the central part of the wing at 24 hAPF. (D) Surface plots of Ds staining at different pupal ages showing the shape of the overall gradient. Scale bars: 100 μm for top image and 5 μm for bottom.DOI:http://dx.doi.org/10.7554/eLife.02893.012
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fig3s3: Orientations of Ds gradient and core proteins correlate in late pupal wing (24 hAPF).(A and B) Images of 24 hAPF and 30 hAPF pupal wings showing Ds staining and Dsh::GFP. (C) Note the strong overlap of Fmi and Ds subcellular localization in the central part of the wing at 24 hAPF. (D) Surface plots of Ds staining at different pupal ages showing the shape of the overall gradient. Scale bars: 100 μm for top image and 5 μm for bottom.DOI:http://dx.doi.org/10.7554/eLife.02893.012

Mentions: (A) Images of 7 hAPF pupal wings showing Ds staining and Dsh::GFP. Note the Ds staining projecting into the central part of the wing. High Ds expression retracts fully into the hinge around or shortly after 24 hAPF (Figure 3—figure supplement 3A,B). (B) A low magnification image of Dsh::GFP at 7 hAPF with overall polarity derived from OrientationJ showing the radial (P/D axis from wing hinge toward wing margin) pattern. Scale bar: 50 μm.


Microtubules provide directional information for core PCP function.

Matis M, Russler-Germain DA, Hu Q, Tomlin CJ, Axelrod JD - Elife (2014)

Orientations of Ds gradient and core proteins correlate in late pupal wing (24 hAPF).(A and B) Images of 24 hAPF and 30 hAPF pupal wings showing Ds staining and Dsh::GFP. (C) Note the strong overlap of Fmi and Ds subcellular localization in the central part of the wing at 24 hAPF. (D) Surface plots of Ds staining at different pupal ages showing the shape of the overall gradient. Scale bars: 100 μm for top image and 5 μm for bottom.DOI:http://dx.doi.org/10.7554/eLife.02893.012
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151085&req=5

fig3s3: Orientations of Ds gradient and core proteins correlate in late pupal wing (24 hAPF).(A and B) Images of 24 hAPF and 30 hAPF pupal wings showing Ds staining and Dsh::GFP. (C) Note the strong overlap of Fmi and Ds subcellular localization in the central part of the wing at 24 hAPF. (D) Surface plots of Ds staining at different pupal ages showing the shape of the overall gradient. Scale bars: 100 μm for top image and 5 μm for bottom.DOI:http://dx.doi.org/10.7554/eLife.02893.012
Mentions: (A) Images of 7 hAPF pupal wings showing Ds staining and Dsh::GFP. Note the Ds staining projecting into the central part of the wing. High Ds expression retracts fully into the hinge around or shortly after 24 hAPF (Figure 3—figure supplement 3A,B). (B) A low magnification image of Dsh::GFP at 7 hAPF with overall polarity derived from OrientationJ showing the radial (P/D axis from wing hinge toward wing margin) pattern. Scale bar: 50 μm.

Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States matism@uni-muenster.de.

Show MeSH
Related in: MedlinePlus