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Microtubules provide directional information for core PCP function.

Matis M, Russler-Germain DA, Hu Q, Tomlin CJ, Axelrod JD - Elife (2014)

Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States matism@uni-muenster.de.

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Related in: MedlinePlus

Orientations of Ds gradient and core proteins correlate in early pupal wing (7 hAPF).(A) Images of 7 hAPF pupal wings showing Ds staining and Dsh::GFP. Note the Ds staining projecting into the central part of the wing. High Ds expression retracts fully into the hinge around or shortly after 24 hAPF (Figure 3—figure supplement 3A,B). (B) A low magnification image of Dsh::GFP at 7 hAPF with overall polarity derived from OrientationJ showing the radial (P/D axis from wing hinge toward wing margin) pattern. Scale bar: 50 μm.DOI:http://dx.doi.org/10.7554/eLife.02893.011
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fig3s2: Orientations of Ds gradient and core proteins correlate in early pupal wing (7 hAPF).(A) Images of 7 hAPF pupal wings showing Ds staining and Dsh::GFP. Note the Ds staining projecting into the central part of the wing. High Ds expression retracts fully into the hinge around or shortly after 24 hAPF (Figure 3—figure supplement 3A,B). (B) A low magnification image of Dsh::GFP at 7 hAPF with overall polarity derived from OrientationJ showing the radial (P/D axis from wing hinge toward wing margin) pattern. Scale bar: 50 μm.DOI:http://dx.doi.org/10.7554/eLife.02893.011

Mentions: Here, our analysis shows that MTs are generally aligned with Ds and Fj gradients from their first appearance in third instar discs (Rogulja et al., 2008), when they emerge on proximal sides of the cell cortex (Figure 1A). Throughout larval wing discs and pupal wings, MT orientation correlates with the direction of Ds and Fj gradients (Figure 1A–D, Figure 1—figure supplement 1). As noted previously (Brittle et al., 2012), in imaginal discs, when the tissue is small and gradients appear to be steeper (Figure 3—figure supplement 1C), marked subcellular asymmetry of Ft localization is observed that substantially overlaps core protein distribution (Figure 3—figure supplement 1A,B). We detect a similar relationship in pupal wings (Figure 3—figure supplements 2 and 3). The steepest regions of the gradients correspond to the most polarized MTs (compare Figure 1D to Figure 3—figure supplement 2A). Therefore, Ds and Fj gradients are appropriately aligned to polarize the MT cytoskeleton and thereby bias core PCP protein polarization from its earliest appearance. These data are consistent with the temporal requirement for Ds in the larval stage (Matakatsu and Blair, 2004; Aigouy et al., 2010).


Microtubules provide directional information for core PCP function.

Matis M, Russler-Germain DA, Hu Q, Tomlin CJ, Axelrod JD - Elife (2014)

Orientations of Ds gradient and core proteins correlate in early pupal wing (7 hAPF).(A) Images of 7 hAPF pupal wings showing Ds staining and Dsh::GFP. Note the Ds staining projecting into the central part of the wing. High Ds expression retracts fully into the hinge around or shortly after 24 hAPF (Figure 3—figure supplement 3A,B). (B) A low magnification image of Dsh::GFP at 7 hAPF with overall polarity derived from OrientationJ showing the radial (P/D axis from wing hinge toward wing margin) pattern. Scale bar: 50 μm.DOI:http://dx.doi.org/10.7554/eLife.02893.011
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151085&req=5

fig3s2: Orientations of Ds gradient and core proteins correlate in early pupal wing (7 hAPF).(A) Images of 7 hAPF pupal wings showing Ds staining and Dsh::GFP. Note the Ds staining projecting into the central part of the wing. High Ds expression retracts fully into the hinge around or shortly after 24 hAPF (Figure 3—figure supplement 3A,B). (B) A low magnification image of Dsh::GFP at 7 hAPF with overall polarity derived from OrientationJ showing the radial (P/D axis from wing hinge toward wing margin) pattern. Scale bar: 50 μm.DOI:http://dx.doi.org/10.7554/eLife.02893.011
Mentions: Here, our analysis shows that MTs are generally aligned with Ds and Fj gradients from their first appearance in third instar discs (Rogulja et al., 2008), when they emerge on proximal sides of the cell cortex (Figure 1A). Throughout larval wing discs and pupal wings, MT orientation correlates with the direction of Ds and Fj gradients (Figure 1A–D, Figure 1—figure supplement 1). As noted previously (Brittle et al., 2012), in imaginal discs, when the tissue is small and gradients appear to be steeper (Figure 3—figure supplement 1C), marked subcellular asymmetry of Ft localization is observed that substantially overlaps core protein distribution (Figure 3—figure supplement 1A,B). We detect a similar relationship in pupal wings (Figure 3—figure supplements 2 and 3). The steepest regions of the gradients correspond to the most polarized MTs (compare Figure 1D to Figure 3—figure supplement 2A). Therefore, Ds and Fj gradients are appropriately aligned to polarize the MT cytoskeleton and thereby bias core PCP protein polarization from its earliest appearance. These data are consistent with the temporal requirement for Ds in the larval stage (Matakatsu and Blair, 2004; Aigouy et al., 2010).

Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States matism@uni-muenster.de.

Show MeSH
Related in: MedlinePlus