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Microtubules provide directional information for core PCP function.

Matis M, Russler-Germain DA, Hu Q, Tomlin CJ, Axelrod JD - Elife (2014)

Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States matism@uni-muenster.de.

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Orientations of Ft, Ds and core proteins correlate in wing disc.(A) Ft staining in third-instar wing pouch. Right panel is a high magnification image of the box in the left panel. (B) Dsh::GFP and Ft localization in a mid third-instar wing disc. Note asymmetric localization of Dsh and Ft that overlap on the P/D boundaries. Scale bar: 2.5 μm. (C) Image of Ds::GFP in third-instar wing disc. On the right and below are vertical section of wing pounch (along red lines) showing the gradient of Ds::GFP along the P/D axis. Scale bar: 2.5 μm.DOI:http://dx.doi.org/10.7554/eLife.02893.010
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fig3s1: Orientations of Ft, Ds and core proteins correlate in wing disc.(A) Ft staining in third-instar wing pouch. Right panel is a high magnification image of the box in the left panel. (B) Dsh::GFP and Ft localization in a mid third-instar wing disc. Note asymmetric localization of Dsh and Ft that overlap on the P/D boundaries. Scale bar: 2.5 μm. (C) Image of Ds::GFP in third-instar wing disc. On the right and below are vertical section of wing pounch (along red lines) showing the gradient of Ds::GFP along the P/D axis. Scale bar: 2.5 μm.DOI:http://dx.doi.org/10.7554/eLife.02893.010

Mentions: Here, our analysis shows that MTs are generally aligned with Ds and Fj gradients from their first appearance in third instar discs (Rogulja et al., 2008), when they emerge on proximal sides of the cell cortex (Figure 1A). Throughout larval wing discs and pupal wings, MT orientation correlates with the direction of Ds and Fj gradients (Figure 1A–D, Figure 1—figure supplement 1). As noted previously (Brittle et al., 2012), in imaginal discs, when the tissue is small and gradients appear to be steeper (Figure 3—figure supplement 1C), marked subcellular asymmetry of Ft localization is observed that substantially overlaps core protein distribution (Figure 3—figure supplement 1A,B). We detect a similar relationship in pupal wings (Figure 3—figure supplements 2 and 3). The steepest regions of the gradients correspond to the most polarized MTs (compare Figure 1D to Figure 3—figure supplement 2A). Therefore, Ds and Fj gradients are appropriately aligned to polarize the MT cytoskeleton and thereby bias core PCP protein polarization from its earliest appearance. These data are consistent with the temporal requirement for Ds in the larval stage (Matakatsu and Blair, 2004; Aigouy et al., 2010).


Microtubules provide directional information for core PCP function.

Matis M, Russler-Germain DA, Hu Q, Tomlin CJ, Axelrod JD - Elife (2014)

Orientations of Ft, Ds and core proteins correlate in wing disc.(A) Ft staining in third-instar wing pouch. Right panel is a high magnification image of the box in the left panel. (B) Dsh::GFP and Ft localization in a mid third-instar wing disc. Note asymmetric localization of Dsh and Ft that overlap on the P/D boundaries. Scale bar: 2.5 μm. (C) Image of Ds::GFP in third-instar wing disc. On the right and below are vertical section of wing pounch (along red lines) showing the gradient of Ds::GFP along the P/D axis. Scale bar: 2.5 μm.DOI:http://dx.doi.org/10.7554/eLife.02893.010
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4151085&req=5

fig3s1: Orientations of Ft, Ds and core proteins correlate in wing disc.(A) Ft staining in third-instar wing pouch. Right panel is a high magnification image of the box in the left panel. (B) Dsh::GFP and Ft localization in a mid third-instar wing disc. Note asymmetric localization of Dsh and Ft that overlap on the P/D boundaries. Scale bar: 2.5 μm. (C) Image of Ds::GFP in third-instar wing disc. On the right and below are vertical section of wing pounch (along red lines) showing the gradient of Ds::GFP along the P/D axis. Scale bar: 2.5 μm.DOI:http://dx.doi.org/10.7554/eLife.02893.010
Mentions: Here, our analysis shows that MTs are generally aligned with Ds and Fj gradients from their first appearance in third instar discs (Rogulja et al., 2008), when they emerge on proximal sides of the cell cortex (Figure 1A). Throughout larval wing discs and pupal wings, MT orientation correlates with the direction of Ds and Fj gradients (Figure 1A–D, Figure 1—figure supplement 1). As noted previously (Brittle et al., 2012), in imaginal discs, when the tissue is small and gradients appear to be steeper (Figure 3—figure supplement 1C), marked subcellular asymmetry of Ft localization is observed that substantially overlaps core protein distribution (Figure 3—figure supplement 1A,B). We detect a similar relationship in pupal wings (Figure 3—figure supplements 2 and 3). The steepest regions of the gradients correspond to the most polarized MTs (compare Figure 1D to Figure 3—figure supplement 2A). Therefore, Ds and Fj gradients are appropriately aligned to polarize the MT cytoskeleton and thereby bias core PCP protein polarization from its earliest appearance. These data are consistent with the temporal requirement for Ds in the larval stage (Matakatsu and Blair, 2004; Aigouy et al., 2010).

Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States matism@uni-muenster.de.

Show MeSH
Related in: MedlinePlus