Microtubules provide directional information for core PCP function.
Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.
Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States firstname.lastname@example.org.Show MeSH
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Mentions: We wished to determine how apical MTs are captured or nucleated at membrane junctions by staining for candidate proteins. EM images showed no association of centrosomes with MTs in non-dividing cells throughout wing development, suggesting that apical MTs are nucleated elsewhere (Figure 2F). We detected the minus-end binding proteins γ-tubulin and Patronin (Stearns and Kirschner, 1994; Goodwin and Vale, 2010) inside the cell, but not at the cell cortex, where they have been observed in other contexts (Meng et al., 2008; Feldman and Priess, 2012; Figure 2—figure supplement 1A). Consistent with this, patronin knockdown (Mummery-Widmer et al., 2009) shows no PCP phenotype. MT associated proteins β-catenin (Armadillo) (Ligon et al., 2001; McCartney et al., 2001), α-catenin (McCartney et al., 2001) and PAR-1 (Doerflinger et al., 2003; Harumoto et al., 2010) are all present symmetrically at the cell cortex but did not show asymmetric localization at the AJs, suggesting they are not involved in apical MT anchoring (Figure 1—figure supplement 1B,C). These results imply that there is an alternative mechanism that nucleates early, apical non-centrosomal MTs.
Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States email@example.com.