Microtubules provide directional information for core PCP function.
Bottom Line: Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton.We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation.Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.
Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States email@example.com.Show MeSH
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Mentions: Apical MT alignment and orientation of core PCP protein domains have been shown to correlate, but have only been examined in several small domains during late pupal wing development (Shimada et al., 2006; Harumoto et al., 2010) (between 14 and 30 hr after puparium formation [APF]) (See also Figure 1—figure supplement 1B–D). If MT alignment provides directional bias for core protein polarization, one should observe a spatiotemporal correlation across the entire wing throughout the time core PCP proteins are polarized.
Affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, United States firstname.lastname@example.org.