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OsSUV3 transgenic rice maintains higher endogenous levels of plant hormones that mitigates adverse effects of salinity and sustains crop productivity.

Sahoo RK, Ansari MW, Tuteja R, Tuteja N - Rice (N Y) (2014)

Bottom Line: However, the Arabidopsis ortholog of SUV3 (AT4G14790) has been shown to be involved in embryo sac development.Previously, we have reported that rice SUV3 functions as DNA and RNA helicase and provides salinity stress tolerance by maintaining photosynthesis and antioxidant machinery.In response to stress, OsSUV3 rice plants maintained plant hormone levels that regulate the expression of several stress-induced genes and reduce adverse effects of salt on plant growth and development and therefore sustains crop productivity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi 110 067, India.

ABSTRACT

Background: The SUV3 (suppressor of Var 3) gene encodes a DNA and RNA helicase, which is localized in the mitochondria. Plant SUV3 has not yet been characterized in detail. However, the Arabidopsis ortholog of SUV3 (AT4G14790) has been shown to be involved in embryo sac development. Previously, we have reported that rice SUV3 functions as DNA and RNA helicase and provides salinity stress tolerance by maintaining photosynthesis and antioxidant machinery. Here, we report further analysis of the transgenic OsSUV3 rice plants under salt stress.

Findings: The transgenic OsSUV3 overexpressing rice T1 lines showed significantly higher endogenous content of plant hormones viz., gibberellic acid (GA3), zeatin (Z) and indole-3-acetic acid (IAA) in leaf, stem and root as compared to wild-type (WT), vector control (VC) and antisense (AS) plants under salt (200 mM NaCl) stress condition. A similar trend of endogenous plant hormones profile was also reflected in the T2 generation of OsSUV3 transgenic rice under defined parameters and stress condition.

Conclusions: In response to stress, OsSUV3 rice plants maintained plant hormone levels that regulate the expression of several stress-induced genes and reduce adverse effects of salt on plant growth and development and therefore sustains crop productivity.

No MeSH data available.


Related in: MedlinePlus

Plant hormones gibberellic acid (GA3), zeatin and indole-3-acetic acid (IAA) profile in the transgenicOsSUV3rice plants under salt stress. Endogenous content (μg/g fw) of GA3, zeatin and IAA in OsSUV3 transgenic rice lines (L1, L2 and L3) of T1 generation (a-c) and T2 generation (d-f) under 200 mM NaCl. The significant difference between the mean values (n = 3) of rice plants (WT, VC and AS) and OsSUV3 overexpressing transgenic rice lines (L1-L3) was determined by one-way analysis of variance (ANOVA) using SPSS 10.0 (SPSS, Inc., now IBM, http://www-01.ibm.com/software/analytics/spss). The WT, VC and transgenic lines at P < 0.05, P < 0.01 and P < 0.001 were considered statistically significant.
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Figure 1: Plant hormones gibberellic acid (GA3), zeatin and indole-3-acetic acid (IAA) profile in the transgenicOsSUV3rice plants under salt stress. Endogenous content (μg/g fw) of GA3, zeatin and IAA in OsSUV3 transgenic rice lines (L1, L2 and L3) of T1 generation (a-c) and T2 generation (d-f) under 200 mM NaCl. The significant difference between the mean values (n = 3) of rice plants (WT, VC and AS) and OsSUV3 overexpressing transgenic rice lines (L1-L3) was determined by one-way analysis of variance (ANOVA) using SPSS 10.0 (SPSS, Inc., now IBM, http://www-01.ibm.com/software/analytics/spss). The WT, VC and transgenic lines at P < 0.05, P < 0.01 and P < 0.001 were considered statistically significant.

Mentions: The OsSUV3 transgenic rice lines viz., Line 1 (L1), Line 2 (L2) and Line 3 (L3) showed higher endogenous content of plant hormones as compared to AS, VC and WT plants (Figure 1a-f). The plant hormones profile of transgenic OsSUV3 rice was assessed upto T2 generation. In T1 generation, GA3 content in leaves of OsSUV3 transgenic rice lines (L1, L2 and L3) was relatively higher (3.07, 3.1 and 3.04 μg/g fw, respectively) as compared to WT (1.54 μg/g fw), VC (1.44 μg/g fw) and AS (1.34 μg/g fw). GA3 content was 2.7, 2.72 and 2.7 μg/g fw in shoots and 1.9, 2.1 and 2.0 μg/g fw in roots of OsSUV3 rice lines (Figure 1a). Zeatin ranged between 0.7-1.27 μg/g fw, whereas control plants showed 0.2-0.52 of zeatin (μg/g fw) in leaf, shoot and roots (Figure 1b). The endogenous level of IAA in leaf, shoot and roots of transgenic rice lines ranged between 1.6-3.1 μg/g fw as opposed to control plants (1.4-1.6 μg/g fw) (Figure 1c). A similar trend of phytohormones (GA3, zeatin and IAA) was also reflected in T2 plants of OsSUV3 transgenic rice lines (L1, L2 and L3) (Figure 1d-f).


OsSUV3 transgenic rice maintains higher endogenous levels of plant hormones that mitigates adverse effects of salinity and sustains crop productivity.

Sahoo RK, Ansari MW, Tuteja R, Tuteja N - Rice (N Y) (2014)

Plant hormones gibberellic acid (GA3), zeatin and indole-3-acetic acid (IAA) profile in the transgenicOsSUV3rice plants under salt stress. Endogenous content (μg/g fw) of GA3, zeatin and IAA in OsSUV3 transgenic rice lines (L1, L2 and L3) of T1 generation (a-c) and T2 generation (d-f) under 200 mM NaCl. The significant difference between the mean values (n = 3) of rice plants (WT, VC and AS) and OsSUV3 overexpressing transgenic rice lines (L1-L3) was determined by one-way analysis of variance (ANOVA) using SPSS 10.0 (SPSS, Inc., now IBM, http://www-01.ibm.com/software/analytics/spss). The WT, VC and transgenic lines at P < 0.05, P < 0.01 and P < 0.001 were considered statistically significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151020&req=5

Figure 1: Plant hormones gibberellic acid (GA3), zeatin and indole-3-acetic acid (IAA) profile in the transgenicOsSUV3rice plants under salt stress. Endogenous content (μg/g fw) of GA3, zeatin and IAA in OsSUV3 transgenic rice lines (L1, L2 and L3) of T1 generation (a-c) and T2 generation (d-f) under 200 mM NaCl. The significant difference between the mean values (n = 3) of rice plants (WT, VC and AS) and OsSUV3 overexpressing transgenic rice lines (L1-L3) was determined by one-way analysis of variance (ANOVA) using SPSS 10.0 (SPSS, Inc., now IBM, http://www-01.ibm.com/software/analytics/spss). The WT, VC and transgenic lines at P < 0.05, P < 0.01 and P < 0.001 were considered statistically significant.
Mentions: The OsSUV3 transgenic rice lines viz., Line 1 (L1), Line 2 (L2) and Line 3 (L3) showed higher endogenous content of plant hormones as compared to AS, VC and WT plants (Figure 1a-f). The plant hormones profile of transgenic OsSUV3 rice was assessed upto T2 generation. In T1 generation, GA3 content in leaves of OsSUV3 transgenic rice lines (L1, L2 and L3) was relatively higher (3.07, 3.1 and 3.04 μg/g fw, respectively) as compared to WT (1.54 μg/g fw), VC (1.44 μg/g fw) and AS (1.34 μg/g fw). GA3 content was 2.7, 2.72 and 2.7 μg/g fw in shoots and 1.9, 2.1 and 2.0 μg/g fw in roots of OsSUV3 rice lines (Figure 1a). Zeatin ranged between 0.7-1.27 μg/g fw, whereas control plants showed 0.2-0.52 of zeatin (μg/g fw) in leaf, shoot and roots (Figure 1b). The endogenous level of IAA in leaf, shoot and roots of transgenic rice lines ranged between 1.6-3.1 μg/g fw as opposed to control plants (1.4-1.6 μg/g fw) (Figure 1c). A similar trend of phytohormones (GA3, zeatin and IAA) was also reflected in T2 plants of OsSUV3 transgenic rice lines (L1, L2 and L3) (Figure 1d-f).

Bottom Line: However, the Arabidopsis ortholog of SUV3 (AT4G14790) has been shown to be involved in embryo sac development.Previously, we have reported that rice SUV3 functions as DNA and RNA helicase and provides salinity stress tolerance by maintaining photosynthesis and antioxidant machinery.In response to stress, OsSUV3 rice plants maintained plant hormone levels that regulate the expression of several stress-induced genes and reduce adverse effects of salt on plant growth and development and therefore sustains crop productivity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi 110 067, India.

ABSTRACT

Background: The SUV3 (suppressor of Var 3) gene encodes a DNA and RNA helicase, which is localized in the mitochondria. Plant SUV3 has not yet been characterized in detail. However, the Arabidopsis ortholog of SUV3 (AT4G14790) has been shown to be involved in embryo sac development. Previously, we have reported that rice SUV3 functions as DNA and RNA helicase and provides salinity stress tolerance by maintaining photosynthesis and antioxidant machinery. Here, we report further analysis of the transgenic OsSUV3 rice plants under salt stress.

Findings: The transgenic OsSUV3 overexpressing rice T1 lines showed significantly higher endogenous content of plant hormones viz., gibberellic acid (GA3), zeatin (Z) and indole-3-acetic acid (IAA) in leaf, stem and root as compared to wild-type (WT), vector control (VC) and antisense (AS) plants under salt (200 mM NaCl) stress condition. A similar trend of endogenous plant hormones profile was also reflected in the T2 generation of OsSUV3 transgenic rice under defined parameters and stress condition.

Conclusions: In response to stress, OsSUV3 rice plants maintained plant hormone levels that regulate the expression of several stress-induced genes and reduce adverse effects of salt on plant growth and development and therefore sustains crop productivity.

No MeSH data available.


Related in: MedlinePlus