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Grouping of Experimental Conditions as an Approach to Evaluate Effects of Extremely Low-Frequency Magnetic Fields on Oxidative Response in in vitro Studies.

Mattsson MO, Simkó M - Front Public Health (2014)

Bottom Line: A large body of literature deals with biological effects of extremely low-frequency magnetic fields (ELF MFs) studied in vitro.Despite the multitude of studies, no coherent picture has evolved regarding the plausibility of effects at low-flux densities or regarding the interaction mechanisms.A response at lower flux densities is seen in certain studies, although not consistently.

View Article: PubMed Central - PubMed

Affiliation: Environmental Resources and Technologies, Department Health and Environment, AIT Austrian Institute of Technology , Tulln , Austria.

ABSTRACT
A large body of literature deals with biological effects of extremely low-frequency magnetic fields (ELF MFs) studied in vitro. Despite the multitude of studies, no coherent picture has evolved regarding the plausibility of effects at low-flux densities or regarding the interaction mechanisms. Here, we propose that ELF MF exposure in vitro causes changes in oxidative status as an early response. We tested this hypothesis by scrutinizing the literature and applying a grouping approach for analyzing relevant biological properties and exposure conditions. A total of 41 scientific original publications were analyzed for this purpose. The conclusion from the work is that ELF MF (modulated or unmodulated) consistently can influence the oxidative status, at or above 1 mT, in a broad range of cell types and independent of exposure duration. A response at lower flux densities is seen in certain studies, although not consistently. Further studies with stringent protocols for sham exposure, blinding, and statistical analysis as well as appropriate positive controls are needed to establish if true dose-relationships for effects on oxidative status exist.

No MeSH data available.


Related in: MedlinePlus

Reactive oxygen species production as a measure of “effect size” after exposure to ELF MF. The direction of change (increase: up and decrease: down) has been considered.
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Figure 6: Reactive oxygen species production as a measure of “effect size” after exposure to ELF MF. The direction of change (increase: up and decrease: down) has been considered.

Mentions: A good measure of the “effect size” is the most common endpoint among the investigated oxidative responses, namely, superoxide radical anion and/or ROS production. However, the effect size is not amenable for grouping purposes, since it is only one parameter. In 18 publications, superoxide radical anion and/or ROS production were reported positive effects. Of these, 17 showed a change between 30 and 90% and one publication was not reporting the size of the effect. Several investigators analyzed this effect after more than one exposure/co-exposure condition. In total, 20 observations showed an increase (up to 90%) and 4 a decrease (20–40%) and 1 showed no effect due to a certain condition in superoxide radical anion and/or ROS release. However, out of these 25 “datasets,” only four investigations reported a change between 50 and 90%. All other data showed a change in superoxide radical anion and/or ROS production at a level of 30–50% (Figure 6).


Grouping of Experimental Conditions as an Approach to Evaluate Effects of Extremely Low-Frequency Magnetic Fields on Oxidative Response in in vitro Studies.

Mattsson MO, Simkó M - Front Public Health (2014)

Reactive oxygen species production as a measure of “effect size” after exposure to ELF MF. The direction of change (increase: up and decrease: down) has been considered.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151017&req=5

Figure 6: Reactive oxygen species production as a measure of “effect size” after exposure to ELF MF. The direction of change (increase: up and decrease: down) has been considered.
Mentions: A good measure of the “effect size” is the most common endpoint among the investigated oxidative responses, namely, superoxide radical anion and/or ROS production. However, the effect size is not amenable for grouping purposes, since it is only one parameter. In 18 publications, superoxide radical anion and/or ROS production were reported positive effects. Of these, 17 showed a change between 30 and 90% and one publication was not reporting the size of the effect. Several investigators analyzed this effect after more than one exposure/co-exposure condition. In total, 20 observations showed an increase (up to 90%) and 4 a decrease (20–40%) and 1 showed no effect due to a certain condition in superoxide radical anion and/or ROS release. However, out of these 25 “datasets,” only four investigations reported a change between 50 and 90%. All other data showed a change in superoxide radical anion and/or ROS production at a level of 30–50% (Figure 6).

Bottom Line: A large body of literature deals with biological effects of extremely low-frequency magnetic fields (ELF MFs) studied in vitro.Despite the multitude of studies, no coherent picture has evolved regarding the plausibility of effects at low-flux densities or regarding the interaction mechanisms.A response at lower flux densities is seen in certain studies, although not consistently.

View Article: PubMed Central - PubMed

Affiliation: Environmental Resources and Technologies, Department Health and Environment, AIT Austrian Institute of Technology , Tulln , Austria.

ABSTRACT
A large body of literature deals with biological effects of extremely low-frequency magnetic fields (ELF MFs) studied in vitro. Despite the multitude of studies, no coherent picture has evolved regarding the plausibility of effects at low-flux densities or regarding the interaction mechanisms. Here, we propose that ELF MF exposure in vitro causes changes in oxidative status as an early response. We tested this hypothesis by scrutinizing the literature and applying a grouping approach for analyzing relevant biological properties and exposure conditions. A total of 41 scientific original publications were analyzed for this purpose. The conclusion from the work is that ELF MF (modulated or unmodulated) consistently can influence the oxidative status, at or above 1 mT, in a broad range of cell types and independent of exposure duration. A response at lower flux densities is seen in certain studies, although not consistently. Further studies with stringent protocols for sham exposure, blinding, and statistical analysis as well as appropriate positive controls are needed to establish if true dose-relationships for effects on oxidative status exist.

No MeSH data available.


Related in: MedlinePlus