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Proteomic profiling of serum from patients with tuberculosis.

Song SH, Han M, Choi YS, Dan KS, Yang MG, Song J, Park SS, Lee JH - Ann Lab Med (2014)

Bottom Line: Peptides from alpha-1-antitrypsin and antithrombin III increased in TB patients and showed a high variable importance in the projection scores and coefficient in partial least square discriminant analysis.Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants.Alpha-1-antitrypsin levels may aid in the diagnosis of TB.

View Article: PubMed Central - PubMed

Affiliation: Clinical Proteomics Laboratory, Seoul National University Hospital, Seoul, Korea. ; Biomedical Research Institute and Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea.

ABSTRACT

Background: Effective treatment and monitoring of tuberculosis (TB) requires biomarkers that can be easily evaluated in blood samples. The aim of this study was to analyze the serum proteome of patients with TB and to identify protein biomarkers for TB.

Methods: Serum samples from 26 TB patients and 31 controls were analyzed by using nano-flow ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in data-independent mode, and protein and peptide amounts were calculated by using a label-free quantitative approach. The generated data were analyzed by using principal component analysis and partial least squares discriminant analysis, a multivariate statistical method.

Results: Of more than 500 proteins identified, alpha-1-antitrypsin was the most discriminative, which was 4.4 times higher in TB patients than in controls. Peptides from alpha-1-antitrypsin and antithrombin III increased in TB patients and showed a high variable importance in the projection scores and coefficient in partial least square discriminant analysis.

Conclusions: Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants. Alpha-1-antitrypsin levels may aid in the diagnosis of TB.

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Related in: MedlinePlus

ROC curves of the proteins (A) and peptides (B) that were significantly higher in tuberculosis patients than in controls. (A) Alpha-1-antitrypsin (P01009) was best able to diagnose tuberculosis patients. The tryptic peptide VFSNGADLSGVTEEAPLKLSK, digested from alpha-1-antitrypsin, performed better than EQLQDMGLVDLFSPEKSK, digested from antithrombin III.
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Figure 3: ROC curves of the proteins (A) and peptides (B) that were significantly higher in tuberculosis patients than in controls. (A) Alpha-1-antitrypsin (P01009) was best able to diagnose tuberculosis patients. The tryptic peptide VFSNGADLSGVTEEAPLKLSK, digested from alpha-1-antitrypsin, performed better than EQLQDMGLVDLFSPEKSK, digested from antithrombin III.

Mentions: The discriminative power of proteins and peptides whose levels differed significantly in TB patients and controls was evaluated by ROC analysis. As shown in Fig. 3, alpha-1-antitrypsin (P01009) performed better than complement component C6 flags precursor (P13671). The areas under the curve (AUC) of the two proteins were 0.947 (0.883-1.000) and 0.681 (0.507-0.856), respectively. When the amounts of the two proteins were summed in ROC analysis, the AUC did not exceed that of alpha-1-antitrypsin. The remaining proteins did not show larger AUC than alpha-1-antitrypsin.


Proteomic profiling of serum from patients with tuberculosis.

Song SH, Han M, Choi YS, Dan KS, Yang MG, Song J, Park SS, Lee JH - Ann Lab Med (2014)

ROC curves of the proteins (A) and peptides (B) that were significantly higher in tuberculosis patients than in controls. (A) Alpha-1-antitrypsin (P01009) was best able to diagnose tuberculosis patients. The tryptic peptide VFSNGADLSGVTEEAPLKLSK, digested from alpha-1-antitrypsin, performed better than EQLQDMGLVDLFSPEKSK, digested from antithrombin III.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4151002&req=5

Figure 3: ROC curves of the proteins (A) and peptides (B) that were significantly higher in tuberculosis patients than in controls. (A) Alpha-1-antitrypsin (P01009) was best able to diagnose tuberculosis patients. The tryptic peptide VFSNGADLSGVTEEAPLKLSK, digested from alpha-1-antitrypsin, performed better than EQLQDMGLVDLFSPEKSK, digested from antithrombin III.
Mentions: The discriminative power of proteins and peptides whose levels differed significantly in TB patients and controls was evaluated by ROC analysis. As shown in Fig. 3, alpha-1-antitrypsin (P01009) performed better than complement component C6 flags precursor (P13671). The areas under the curve (AUC) of the two proteins were 0.947 (0.883-1.000) and 0.681 (0.507-0.856), respectively. When the amounts of the two proteins were summed in ROC analysis, the AUC did not exceed that of alpha-1-antitrypsin. The remaining proteins did not show larger AUC than alpha-1-antitrypsin.

Bottom Line: Peptides from alpha-1-antitrypsin and antithrombin III increased in TB patients and showed a high variable importance in the projection scores and coefficient in partial least square discriminant analysis.Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants.Alpha-1-antitrypsin levels may aid in the diagnosis of TB.

View Article: PubMed Central - PubMed

Affiliation: Clinical Proteomics Laboratory, Seoul National University Hospital, Seoul, Korea. ; Biomedical Research Institute and Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea.

ABSTRACT

Background: Effective treatment and monitoring of tuberculosis (TB) requires biomarkers that can be easily evaluated in blood samples. The aim of this study was to analyze the serum proteome of patients with TB and to identify protein biomarkers for TB.

Methods: Serum samples from 26 TB patients and 31 controls were analyzed by using nano-flow ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in data-independent mode, and protein and peptide amounts were calculated by using a label-free quantitative approach. The generated data were analyzed by using principal component analysis and partial least squares discriminant analysis, a multivariate statistical method.

Results: Of more than 500 proteins identified, alpha-1-antitrypsin was the most discriminative, which was 4.4 times higher in TB patients than in controls. Peptides from alpha-1-antitrypsin and antithrombin III increased in TB patients and showed a high variable importance in the projection scores and coefficient in partial least square discriminant analysis.

Conclusions: Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants. Alpha-1-antitrypsin levels may aid in the diagnosis of TB.

Show MeSH
Related in: MedlinePlus