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MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma.

Sun Z, Meng C, Wang S, Zhou N, Guan M, Bai C, Lu S, Han Q, Zhao RC - BMC Cancer (2014)

Bottom Line: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines.Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, People's Republic of China. hanqinhanqin@126.com.

ABSTRACT

Background: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.

Methods: Transwell chambers (8-uM pore size; Costar) were used in the in vitro migration and invison anssay. Dual luciferase reporter gene construct and Dual luciferase reporter assay to identify the target of miR-1246. CADM1 expression was evaluated by immunohistochemistric staining. The clinical manifestations, treatments and survival were collected for statistical analysis.

Results: Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines. Bioinformatics and luciferase reporter assay revealed that miR-1246 specifically targeted the 3'-UTR of Cell adhesion molecule 1 and regulated its expression. Down-regulation of CADM1 enhanced migration and invasion of HCC cell lines. Furthermore, in tumor tissues obtained from liver cancer patients, the expression of miR-1246 was negatively correlated with CADM1 and the high expression of miR-1246 combined with low expression of CADM1 might serve as a risk factor for stage1 liver cancer patients.

Conclusions: Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.

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Related in: MedlinePlus

Influence of different factors on DFS of the patients. (A) Cox assay was used to examine the influence of different factors on DFS of the patients; (B) the DFS of all patients; (C) the influence of miR-1246 expression on DFS of stage I patients (p = 0.143); (D) the relationship between expression of and DFS in stage I patients (p = 0.039).
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Fig6: Influence of different factors on DFS of the patients. (A) Cox assay was used to examine the influence of different factors on DFS of the patients; (B) the DFS of all patients; (C) the influence of miR-1246 expression on DFS of stage I patients (p = 0.143); (D) the relationship between expression of and DFS in stage I patients (p = 0.039).

Mentions: From 2009 to 2010, 91 patients with liver cancer got surgery in Peking Union Medical College hospital and among them only 38 had follow-up data for statistical analysis. By June 30th, 2013, 19 patients had recurrence and 11 patients died. So we didn’t perform OS. Using cox assay to analyze the influence of multiple factors on DFS such as ECOG score, serum AFP, TNM, pathological differentiation and miR-1246 and CADM1 expression, we found only TNM and pathological differentiation were statistically significantly correlated with DFS (Figure 6A, B), Kaplan-Meier assay also confirmed this result (Additional file 1: Table S7 and 8).


MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma.

Sun Z, Meng C, Wang S, Zhou N, Guan M, Bai C, Lu S, Han Q, Zhao RC - BMC Cancer (2014)

Influence of different factors on DFS of the patients. (A) Cox assay was used to examine the influence of different factors on DFS of the patients; (B) the DFS of all patients; (C) the influence of miR-1246 expression on DFS of stage I patients (p = 0.143); (D) the relationship between expression of and DFS in stage I patients (p = 0.039).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4150976&req=5

Fig6: Influence of different factors on DFS of the patients. (A) Cox assay was used to examine the influence of different factors on DFS of the patients; (B) the DFS of all patients; (C) the influence of miR-1246 expression on DFS of stage I patients (p = 0.143); (D) the relationship between expression of and DFS in stage I patients (p = 0.039).
Mentions: From 2009 to 2010, 91 patients with liver cancer got surgery in Peking Union Medical College hospital and among them only 38 had follow-up data for statistical analysis. By June 30th, 2013, 19 patients had recurrence and 11 patients died. So we didn’t perform OS. Using cox assay to analyze the influence of multiple factors on DFS such as ECOG score, serum AFP, TNM, pathological differentiation and miR-1246 and CADM1 expression, we found only TNM and pathological differentiation were statistically significantly correlated with DFS (Figure 6A, B), Kaplan-Meier assay also confirmed this result (Additional file 1: Table S7 and 8).

Bottom Line: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines.Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, People's Republic of China. hanqinhanqin@126.com.

ABSTRACT

Background: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.

Methods: Transwell chambers (8-uM pore size; Costar) were used in the in vitro migration and invison anssay. Dual luciferase reporter gene construct and Dual luciferase reporter assay to identify the target of miR-1246. CADM1 expression was evaluated by immunohistochemistric staining. The clinical manifestations, treatments and survival were collected for statistical analysis.

Results: Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines. Bioinformatics and luciferase reporter assay revealed that miR-1246 specifically targeted the 3'-UTR of Cell adhesion molecule 1 and regulated its expression. Down-regulation of CADM1 enhanced migration and invasion of HCC cell lines. Furthermore, in tumor tissues obtained from liver cancer patients, the expression of miR-1246 was negatively correlated with CADM1 and the high expression of miR-1246 combined with low expression of CADM1 might serve as a risk factor for stage1 liver cancer patients.

Conclusions: Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.

Show MeSH
Related in: MedlinePlus