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MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma.

Sun Z, Meng C, Wang S, Zhou N, Guan M, Bai C, Lu S, Han Q, Zhao RC - BMC Cancer (2014)

Bottom Line: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines.Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, People's Republic of China. hanqinhanqin@126.com.

ABSTRACT

Background: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.

Methods: Transwell chambers (8-uM pore size; Costar) were used in the in vitro migration and invison anssay. Dual luciferase reporter gene construct and Dual luciferase reporter assay to identify the target of miR-1246. CADM1 expression was evaluated by immunohistochemistric staining. The clinical manifestations, treatments and survival were collected for statistical analysis.

Results: Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines. Bioinformatics and luciferase reporter assay revealed that miR-1246 specifically targeted the 3'-UTR of Cell adhesion molecule 1 and regulated its expression. Down-regulation of CADM1 enhanced migration and invasion of HCC cell lines. Furthermore, in tumor tissues obtained from liver cancer patients, the expression of miR-1246 was negatively correlated with CADM1 and the high expression of miR-1246 combined with low expression of CADM1 might serve as a risk factor for stage1 liver cancer patients.

Conclusions: Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.

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Related in: MedlinePlus

Immunohistochemistry of CADM1. (A) negative; (B) positive +; (C) positive++.
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Fig5: Immunohistochemistry of CADM1. (A) negative; (B) positive +; (C) positive++.

Mentions: We obtained clinical samples from 38 patients of liver cancer. Additional file 1: TableS5 showed the clinical characteristics of these patients. To investigate the correlation between miR-1246 and CADM1 expression, we analyzed these liver cancer tissue samples by RT-PCR and immunohistochemisty for miR-1246 and CADM1, respectively. Figure 5 showed the characteristic expression of CADM1. The results demonstrated that patients with high miR-1246 expression were CADM1 negative while patients with low miR-1246 expression were CADM1 positive (p = 0.003 Additional file 1: Table S6). The expression of miR-1246 was negatively correlated to CADM1, which was of statistical significance.Figure 5


MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma.

Sun Z, Meng C, Wang S, Zhou N, Guan M, Bai C, Lu S, Han Q, Zhao RC - BMC Cancer (2014)

Immunohistochemistry of CADM1. (A) negative; (B) positive +; (C) positive++.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4150976&req=5

Fig5: Immunohistochemistry of CADM1. (A) negative; (B) positive +; (C) positive++.
Mentions: We obtained clinical samples from 38 patients of liver cancer. Additional file 1: TableS5 showed the clinical characteristics of these patients. To investigate the correlation between miR-1246 and CADM1 expression, we analyzed these liver cancer tissue samples by RT-PCR and immunohistochemisty for miR-1246 and CADM1, respectively. Figure 5 showed the characteristic expression of CADM1. The results demonstrated that patients with high miR-1246 expression were CADM1 negative while patients with low miR-1246 expression were CADM1 positive (p = 0.003 Additional file 1: Table S6). The expression of miR-1246 was negatively correlated to CADM1, which was of statistical significance.Figure 5

Bottom Line: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines.Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, People's Republic of China. hanqinhanqin@126.com.

ABSTRACT

Background: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line.

Methods: Transwell chambers (8-uM pore size; Costar) were used in the in vitro migration and invison anssay. Dual luciferase reporter gene construct and Dual luciferase reporter assay to identify the target of miR-1246. CADM1 expression was evaluated by immunohistochemistric staining. The clinical manifestations, treatments and survival were collected for statistical analysis.

Results: Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines. Bioinformatics and luciferase reporter assay revealed that miR-1246 specifically targeted the 3'-UTR of Cell adhesion molecule 1 and regulated its expression. Down-regulation of CADM1 enhanced migration and invasion of HCC cell lines. Furthermore, in tumor tissues obtained from liver cancer patients, the expression of miR-1246 was negatively correlated with CADM1 and the high expression of miR-1246 combined with low expression of CADM1 might serve as a risk factor for stage1 liver cancer patients.

Conclusions: Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells.

Show MeSH
Related in: MedlinePlus