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Twist 1 regulates the expression of PPARγ during hormone-induced 3T3-L1 preadipocyte differentiation: a possible role in obesity and associated diseases.

Ma W, Lu S, Sun T, Wang X, Ma Y, Zhang X, Zhao R, Wang Y - Lipids Health Dis (2014)

Bottom Line: Twist 1 mRNA and protein levels were reduced in diet-induced obese mice and rats and in obese humans.Retroviral interference of Twist 1 expression did not significantly impair lipid formation; however, retroviral interference induced PPARγ mRNA and protein expression on day 4 of differentiation induction.Adipokine array analyses revealed increased secretion of CXCR4 (19.55-fold), VEGFR1 (92.13-fold), L-21 R (63.55-fold), and IL-12 R beta 1 (59.66-fold) and decreased secretion of VEGFR3 (0.01-fold), TSLP R (0.071-fold), MIP-1 gamma (0.069-fold), TNF RI/TNFRSF1A (0.09-fold), and MFG-E8 (0.06-fold).

View Article: PubMed Central - PubMed

Affiliation: Medical Research & Laboratory Diagnostic Center, Jinan Center Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P,R, China. mwsqianyi@163.com.

ABSTRACT

Background: Twist 1 is highly expressed in adipose tissue and has been associated with obesity and related disorders. However, the molecular function of Twist 1 in adipose tissue is unclear. Twist 1 has been implicated in cell lineage determination and differentiation. Therefore, we investigated both the role of Twist 1 in adipocyte precursor mobilization and the relationship of Twist 1 with other molecular determinants of adipocyte differentiation.

Methods: We examined Twist 1 mRNA and protein expression in subcutaneous adipose tissues from diet-induced obese C57/BL6 mice and Wistar rats and in obese patients undergoing liposuction or adipose transplant surgeries. Twist 1 expression was measured on days 0, 2, 4, 8, and 12 of 3T3-L1 differentiation in vitro. The role of Twist 1 in adipogenesis was explored using retroviral interference of Twist 1 expression. Adipokine secretion was evaluated using a RayBio® Biotin Label-based Adipokine Array.

Results: Twist 1 mRNA and protein levels were reduced in diet-induced obese mice and rats and in obese humans. Twist 1 was upregulated during 3T3-L1 preadipocyte differentiation in vitro, beginning from the fourth day of differentiation induction. Retroviral interference of Twist 1 expression did not significantly impair lipid formation; however, retroviral interference induced PPARγ mRNA and protein expression on day 4 of differentiation induction. Adipokine array analyses revealed increased secretion of CXCR4 (19.55-fold), VEGFR1 (92.13-fold), L-21 R (63.55-fold), and IL-12 R beta 1 (59.66-fold) and decreased secretion of VEGFR3 (0.01-fold), TSLP R (0.071-fold), MIP-1 gamma (0.069-fold), TNF RI/TNFRSF1A (0.09-fold), and MFG-E8 (0.06-fold).

Conclusions: Twist 1 is a regulator of adipocyte gene expression although it is not likely to regulate differentiation. We identified PPARγ as a potential target of Twist 1 and found variation in the secretion of multiple adipokines, which might indicate a prospective mechanism linking Twist 1 expression with obesity or associated diseases.

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Related in: MedlinePlus

Twist 1 transcription was downregulated in the adipose tissue of obese subjects. (A/B) An obesity model was established in C57/BL6J mice that were fed a high-fat diet for 14 weeks. Twist 1 transcription was downregulated significantly in subcutaneous adipose tissue compared with its level in the control groups (*P < 0.05 vs. control). (C/D) An obesity model was established in Wistar rats that were fed a high-fat diet, and a reduced Twist 1 mRNA level was observed in subcutaneous adipose tissue (*P < 0.05 vs. control). (E/F) The level of Twist 1 transcription in the subcutaneous adipose tissues of individuals with a BMI ≥ 30 was lower than in non-obese individuals (*P < 0.05 vs. 20 < BMI < 25 group).
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Fig2: Twist 1 transcription was downregulated in the adipose tissue of obese subjects. (A/B) An obesity model was established in C57/BL6J mice that were fed a high-fat diet for 14 weeks. Twist 1 transcription was downregulated significantly in subcutaneous adipose tissue compared with its level in the control groups (*P < 0.05 vs. control). (C/D) An obesity model was established in Wistar rats that were fed a high-fat diet, and a reduced Twist 1 mRNA level was observed in subcutaneous adipose tissue (*P < 0.05 vs. control). (E/F) The level of Twist 1 transcription in the subcutaneous adipose tissues of individuals with a BMI ≥ 30 was lower than in non-obese individuals (*P < 0.05 vs. 20 < BMI < 25 group).

Mentions: In both C57/BL6 mice (Figure 2A/B) and Wistar rats (Figure 2C/D), Twist 1 gene transcription was significantly decreased in obese animals compared with non-obese animals. A semi-quantitative analysis using Image J software verified this decrease (P < 0.05). We further confirmed this decrease in Twist 1 in clinically obese patients with a BMI ≥ 30 compared with non-obese patients (Figure 2E/F) (P < 0.05).Figure 2


Twist 1 regulates the expression of PPARγ during hormone-induced 3T3-L1 preadipocyte differentiation: a possible role in obesity and associated diseases.

Ma W, Lu S, Sun T, Wang X, Ma Y, Zhang X, Zhao R, Wang Y - Lipids Health Dis (2014)

Twist 1 transcription was downregulated in the adipose tissue of obese subjects. (A/B) An obesity model was established in C57/BL6J mice that were fed a high-fat diet for 14 weeks. Twist 1 transcription was downregulated significantly in subcutaneous adipose tissue compared with its level in the control groups (*P < 0.05 vs. control). (C/D) An obesity model was established in Wistar rats that were fed a high-fat diet, and a reduced Twist 1 mRNA level was observed in subcutaneous adipose tissue (*P < 0.05 vs. control). (E/F) The level of Twist 1 transcription in the subcutaneous adipose tissues of individuals with a BMI ≥ 30 was lower than in non-obese individuals (*P < 0.05 vs. 20 < BMI < 25 group).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4150960&req=5

Fig2: Twist 1 transcription was downregulated in the adipose tissue of obese subjects. (A/B) An obesity model was established in C57/BL6J mice that were fed a high-fat diet for 14 weeks. Twist 1 transcription was downregulated significantly in subcutaneous adipose tissue compared with its level in the control groups (*P < 0.05 vs. control). (C/D) An obesity model was established in Wistar rats that were fed a high-fat diet, and a reduced Twist 1 mRNA level was observed in subcutaneous adipose tissue (*P < 0.05 vs. control). (E/F) The level of Twist 1 transcription in the subcutaneous adipose tissues of individuals with a BMI ≥ 30 was lower than in non-obese individuals (*P < 0.05 vs. 20 < BMI < 25 group).
Mentions: In both C57/BL6 mice (Figure 2A/B) and Wistar rats (Figure 2C/D), Twist 1 gene transcription was significantly decreased in obese animals compared with non-obese animals. A semi-quantitative analysis using Image J software verified this decrease (P < 0.05). We further confirmed this decrease in Twist 1 in clinically obese patients with a BMI ≥ 30 compared with non-obese patients (Figure 2E/F) (P < 0.05).Figure 2

Bottom Line: Twist 1 mRNA and protein levels were reduced in diet-induced obese mice and rats and in obese humans.Retroviral interference of Twist 1 expression did not significantly impair lipid formation; however, retroviral interference induced PPARγ mRNA and protein expression on day 4 of differentiation induction.Adipokine array analyses revealed increased secretion of CXCR4 (19.55-fold), VEGFR1 (92.13-fold), L-21 R (63.55-fold), and IL-12 R beta 1 (59.66-fold) and decreased secretion of VEGFR3 (0.01-fold), TSLP R (0.071-fold), MIP-1 gamma (0.069-fold), TNF RI/TNFRSF1A (0.09-fold), and MFG-E8 (0.06-fold).

View Article: PubMed Central - PubMed

Affiliation: Medical Research & Laboratory Diagnostic Center, Jinan Center Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P,R, China. mwsqianyi@163.com.

ABSTRACT

Background: Twist 1 is highly expressed in adipose tissue and has been associated with obesity and related disorders. However, the molecular function of Twist 1 in adipose tissue is unclear. Twist 1 has been implicated in cell lineage determination and differentiation. Therefore, we investigated both the role of Twist 1 in adipocyte precursor mobilization and the relationship of Twist 1 with other molecular determinants of adipocyte differentiation.

Methods: We examined Twist 1 mRNA and protein expression in subcutaneous adipose tissues from diet-induced obese C57/BL6 mice and Wistar rats and in obese patients undergoing liposuction or adipose transplant surgeries. Twist 1 expression was measured on days 0, 2, 4, 8, and 12 of 3T3-L1 differentiation in vitro. The role of Twist 1 in adipogenesis was explored using retroviral interference of Twist 1 expression. Adipokine secretion was evaluated using a RayBio® Biotin Label-based Adipokine Array.

Results: Twist 1 mRNA and protein levels were reduced in diet-induced obese mice and rats and in obese humans. Twist 1 was upregulated during 3T3-L1 preadipocyte differentiation in vitro, beginning from the fourth day of differentiation induction. Retroviral interference of Twist 1 expression did not significantly impair lipid formation; however, retroviral interference induced PPARγ mRNA and protein expression on day 4 of differentiation induction. Adipokine array analyses revealed increased secretion of CXCR4 (19.55-fold), VEGFR1 (92.13-fold), L-21 R (63.55-fold), and IL-12 R beta 1 (59.66-fold) and decreased secretion of VEGFR3 (0.01-fold), TSLP R (0.071-fold), MIP-1 gamma (0.069-fold), TNF RI/TNFRSF1A (0.09-fold), and MFG-E8 (0.06-fold).

Conclusions: Twist 1 is a regulator of adipocyte gene expression although it is not likely to regulate differentiation. We identified PPARγ as a potential target of Twist 1 and found variation in the secretion of multiple adipokines, which might indicate a prospective mechanism linking Twist 1 expression with obesity or associated diseases.

Show MeSH
Related in: MedlinePlus