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Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom.

Walker T, Jeffries CL, Mansfield KL, Johnson N - Parasit Vectors (2014)

Bottom Line: Mosquitoes are highly effective vectors for transmission of human and animal pathogens.Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission.Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes.

View Article: PubMed Central - PubMed

Affiliation: Animal Health and Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK. Claire.Jeffries@lshtm.ac.uk.

ABSTRACT
Mosquitoes are highly effective vectors for transmission of human and animal pathogens. Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission. Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes. This review provides a brief overview of the development of mosquito cell lines, methods of isolation, their availability and application for investigating insect-virus interactions.

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Related in: MedlinePlus

Generation of anAedes vexanscell line of UK origin using embryos as the source material. The first passage (P) was undertaken 14 days (d) after adding crushed embryos to cell media and P2 was undertaken 7d after P1 to allow cells to adhere. Routine passage of the cell line was undertaken from P3 by transferring 20% cells in media to 80% media. A) P1 after 14d showing formation of a high density monolayer. B) P2 after 7d showing the start of a monolayer forming. C) P3 after 1d and D) P3 after 4d showing growth of the cell line.
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Fig2: Generation of anAedes vexanscell line of UK origin using embryos as the source material. The first passage (P) was undertaken 14 days (d) after adding crushed embryos to cell media and P2 was undertaken 7d after P1 to allow cells to adhere. Routine passage of the cell line was undertaken from P3 by transferring 20% cells in media to 80% media. A) P1 after 14d showing formation of a high density monolayer. B) P2 after 7d showing the start of a monolayer forming. C) P3 after 1d and D) P3 after 4d showing growth of the cell line.

Mentions: Although several mosquito cell lines have been established from major mosquito disease vectors such as An. gambiae (malaria) and Ae. aegypti (dengue), further cell lines are needed for native temperate mosquito species that are considered potential vectors of arboviruses. Sporadic WNV outbreaks have occurred in warmer regions of Europe for the past 20 years and could occur more frequently as a result of climate change [63]. Cx. pipiens mosquitoes are widespread throughout Europe, and are critical vectors of WNV in the USA and southern Europe. Currently available cell lines, such as C6/36 and RML-12, are high passage cell lines, which may not be susceptible to many arboviruses that are considered a threat to countries such as the UK, including WNV. Ae. vexans is a vector of Tahyna virus and is the most common mosquito in Europe, but is relatively rare in the UK. This species has also been shown to be a competent vector of both WNV and RVFV under laboratory conditions. Preliminary experiments have been undertaken to generate an Ae. vexans cell line from a colony of UK origin. As shown in FigureĀ 2, evidence for cell line growth and adhesion to form a monolayer has been observed in early passages. This cell line will be used to determine the initial virus-vector interactions of Ae. vexans to a diverse range of arboviruses that are considered to be a potential threat to the UK and help to inform future in vivo vector competence studies.Figure 2


Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom.

Walker T, Jeffries CL, Mansfield KL, Johnson N - Parasit Vectors (2014)

Generation of anAedes vexanscell line of UK origin using embryos as the source material. The first passage (P) was undertaken 14 days (d) after adding crushed embryos to cell media and P2 was undertaken 7d after P1 to allow cells to adhere. Routine passage of the cell line was undertaken from P3 by transferring 20% cells in media to 80% media. A) P1 after 14d showing formation of a high density monolayer. B) P2 after 7d showing the start of a monolayer forming. C) P3 after 1d and D) P3 after 4d showing growth of the cell line.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4150944&req=5

Fig2: Generation of anAedes vexanscell line of UK origin using embryos as the source material. The first passage (P) was undertaken 14 days (d) after adding crushed embryos to cell media and P2 was undertaken 7d after P1 to allow cells to adhere. Routine passage of the cell line was undertaken from P3 by transferring 20% cells in media to 80% media. A) P1 after 14d showing formation of a high density monolayer. B) P2 after 7d showing the start of a monolayer forming. C) P3 after 1d and D) P3 after 4d showing growth of the cell line.
Mentions: Although several mosquito cell lines have been established from major mosquito disease vectors such as An. gambiae (malaria) and Ae. aegypti (dengue), further cell lines are needed for native temperate mosquito species that are considered potential vectors of arboviruses. Sporadic WNV outbreaks have occurred in warmer regions of Europe for the past 20 years and could occur more frequently as a result of climate change [63]. Cx. pipiens mosquitoes are widespread throughout Europe, and are critical vectors of WNV in the USA and southern Europe. Currently available cell lines, such as C6/36 and RML-12, are high passage cell lines, which may not be susceptible to many arboviruses that are considered a threat to countries such as the UK, including WNV. Ae. vexans is a vector of Tahyna virus and is the most common mosquito in Europe, but is relatively rare in the UK. This species has also been shown to be a competent vector of both WNV and RVFV under laboratory conditions. Preliminary experiments have been undertaken to generate an Ae. vexans cell line from a colony of UK origin. As shown in FigureĀ 2, evidence for cell line growth and adhesion to form a monolayer has been observed in early passages. This cell line will be used to determine the initial virus-vector interactions of Ae. vexans to a diverse range of arboviruses that are considered to be a potential threat to the UK and help to inform future in vivo vector competence studies.Figure 2

Bottom Line: Mosquitoes are highly effective vectors for transmission of human and animal pathogens.Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission.Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes.

View Article: PubMed Central - PubMed

Affiliation: Animal Health and Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK. Claire.Jeffries@lshtm.ac.uk.

ABSTRACT
Mosquitoes are highly effective vectors for transmission of human and animal pathogens. Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission. Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes. This review provides a brief overview of the development of mosquito cell lines, methods of isolation, their availability and application for investigating insect-virus interactions.

Show MeSH
Related in: MedlinePlus