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Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom.

Walker T, Jeffries CL, Mansfield KL, Johnson N - Parasit Vectors (2014)

Bottom Line: Mosquitoes are highly effective vectors for transmission of human and animal pathogens.Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission.Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes.

View Article: PubMed Central - PubMed

Affiliation: Animal Health and Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK. Claire.Jeffries@lshtm.ac.uk.

ABSTRACT
Mosquitoes are highly effective vectors for transmission of human and animal pathogens. Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission. Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes. This review provides a brief overview of the development of mosquito cell lines, methods of isolation, their availability and application for investigating insect-virus interactions.

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Related in: MedlinePlus

An illustration of the lifecycle of mosquitoes and potential sources of cell lines.
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Fig1: An illustration of the lifecycle of mosquitoes and potential sources of cell lines.

Mentions: The mosquito lifecycle in its simplest form is composed of a series of life stages beginning with eggs laid on or near water that hatch after a number of days into larvae. The larvae obtain nutrition predominantly through filter-feeding but predation on other larvae and small invertebrates also occurs for certain species. Mosquitoes develop through four instar phases, to form non-feeding pupae, which metamorphose into adults (FigureĀ 1). Adult males emerge first, followed by females and mating occurs when females are 2-3 days old. Whilst both males and females can derive nutrition from nectar, in most species females require a blood meal to promote egg development through the acquisition of protein and iron from blood. This provides the opportunity for pathogen transmission, particularly as female mosquitoes take multiple feeds during their lifecycle. Cells can be obtained from each of these developmental stages to generate cell lines appropriate for each experimental approach.Figure 1


Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom.

Walker T, Jeffries CL, Mansfield KL, Johnson N - Parasit Vectors (2014)

An illustration of the lifecycle of mosquitoes and potential sources of cell lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4150944&req=5

Fig1: An illustration of the lifecycle of mosquitoes and potential sources of cell lines.
Mentions: The mosquito lifecycle in its simplest form is composed of a series of life stages beginning with eggs laid on or near water that hatch after a number of days into larvae. The larvae obtain nutrition predominantly through filter-feeding but predation on other larvae and small invertebrates also occurs for certain species. Mosquitoes develop through four instar phases, to form non-feeding pupae, which metamorphose into adults (FigureĀ 1). Adult males emerge first, followed by females and mating occurs when females are 2-3 days old. Whilst both males and females can derive nutrition from nectar, in most species females require a blood meal to promote egg development through the acquisition of protein and iron from blood. This provides the opportunity for pathogen transmission, particularly as female mosquitoes take multiple feeds during their lifecycle. Cells can be obtained from each of these developmental stages to generate cell lines appropriate for each experimental approach.Figure 1

Bottom Line: Mosquitoes are highly effective vectors for transmission of human and animal pathogens.Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission.Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes.

View Article: PubMed Central - PubMed

Affiliation: Animal Health and Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK. Claire.Jeffries@lshtm.ac.uk.

ABSTRACT
Mosquitoes are highly effective vectors for transmission of human and animal pathogens. Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission. Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes. This review provides a brief overview of the development of mosquito cell lines, methods of isolation, their availability and application for investigating insect-virus interactions.

Show MeSH
Related in: MedlinePlus