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MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2.

Cheng Z, Ma R, Tan W, Zhang L - Exp. Mol. Med. (2014)

Bottom Line: MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells.Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion.FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, ZhongNan Hospital of WuHan University, WuHan, China.

ABSTRACT
MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells. Here, we showed that miR-152 was downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion. Fibroblast growth factor 2 (FGF2) was confirmed as a direct target of miR-152. FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152. Furthermore, the presence of miR-152 was inversely correlated with FGF2 in NSCLC tissues. Overall, this study demonstrated that miR-152 suppressed the proliferation and invasion of NSCLC cells by downregulating FGF2. These findings provide novel insights with potential therapeutic applications for the treatment of NSCLC.

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Related in: MedlinePlus

Fibroblast growth factor 2 (FGF2) levels were inversely correlated with microRNA-152 (miR-152) in non-small-cell lung cancer (NSCLC) tissues. (a) FGF2 expression levels in 30 pairs of NSCLC tissues and their matched normal tissues were measured by quantitative real-time PCR (qRT-PCR). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. (b) Correlation analysis between FGF2 mRNA levels and miR-152 expression in NSCLC tissues (Spearman's correlation analysis, r=−0.422; P<0.05). *P<0.05 vs control.
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fig7: Fibroblast growth factor 2 (FGF2) levels were inversely correlated with microRNA-152 (miR-152) in non-small-cell lung cancer (NSCLC) tissues. (a) FGF2 expression levels in 30 pairs of NSCLC tissues and their matched normal tissues were measured by quantitative real-time PCR (qRT-PCR). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. (b) Correlation analysis between FGF2 mRNA levels and miR-152 expression in NSCLC tissues (Spearman's correlation analysis, r=−0.422; P<0.05). *P<0.05 vs control.

Mentions: To further explore the relationship between FGF2 and miR-152 in vivo, we examined the expression of FGF2 in 30 pairs of NSCLC tissues and their matched normal tissues using qRT-PCR. We found that FGF2 expression was significantly increased in NSCLC tissues relative to the matched controls (Figure 7a). Moreover, FGF2 was negatively correlated with miR-152 expression in the same NSCLC tissues (Figure 7b). These data further indicate that FGF2 was a target of miR-152 in NSCLC.


MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2.

Cheng Z, Ma R, Tan W, Zhang L - Exp. Mol. Med. (2014)

Fibroblast growth factor 2 (FGF2) levels were inversely correlated with microRNA-152 (miR-152) in non-small-cell lung cancer (NSCLC) tissues. (a) FGF2 expression levels in 30 pairs of NSCLC tissues and their matched normal tissues were measured by quantitative real-time PCR (qRT-PCR). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. (b) Correlation analysis between FGF2 mRNA levels and miR-152 expression in NSCLC tissues (Spearman's correlation analysis, r=−0.422; P<0.05). *P<0.05 vs control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150934&req=5

fig7: Fibroblast growth factor 2 (FGF2) levels were inversely correlated with microRNA-152 (miR-152) in non-small-cell lung cancer (NSCLC) tissues. (a) FGF2 expression levels in 30 pairs of NSCLC tissues and their matched normal tissues were measured by quantitative real-time PCR (qRT-PCR). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. (b) Correlation analysis between FGF2 mRNA levels and miR-152 expression in NSCLC tissues (Spearman's correlation analysis, r=−0.422; P<0.05). *P<0.05 vs control.
Mentions: To further explore the relationship between FGF2 and miR-152 in vivo, we examined the expression of FGF2 in 30 pairs of NSCLC tissues and their matched normal tissues using qRT-PCR. We found that FGF2 expression was significantly increased in NSCLC tissues relative to the matched controls (Figure 7a). Moreover, FGF2 was negatively correlated with miR-152 expression in the same NSCLC tissues (Figure 7b). These data further indicate that FGF2 was a target of miR-152 in NSCLC.

Bottom Line: MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells.Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion.FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, ZhongNan Hospital of WuHan University, WuHan, China.

ABSTRACT
MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells. Here, we showed that miR-152 was downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion. Fibroblast growth factor 2 (FGF2) was confirmed as a direct target of miR-152. FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152. Furthermore, the presence of miR-152 was inversely correlated with FGF2 in NSCLC tissues. Overall, this study demonstrated that miR-152 suppressed the proliferation and invasion of NSCLC cells by downregulating FGF2. These findings provide novel insights with potential therapeutic applications for the treatment of NSCLC.

Show MeSH
Related in: MedlinePlus