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MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2.

Cheng Z, Ma R, Tan W, Zhang L - Exp. Mol. Med. (2014)

Bottom Line: MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells.Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion.FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, ZhongNan Hospital of WuHan University, WuHan, China.

ABSTRACT
MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells. Here, we showed that miR-152 was downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion. Fibroblast growth factor 2 (FGF2) was confirmed as a direct target of miR-152. FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152. Furthermore, the presence of miR-152 was inversely correlated with FGF2 in NSCLC tissues. Overall, this study demonstrated that miR-152 suppressed the proliferation and invasion of NSCLC cells by downregulating FGF2. These findings provide novel insights with potential therapeutic applications for the treatment of NSCLC.

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Related in: MedlinePlus

Fibroblast growth factor 2 (FGF2) overexpression partially attenuated the tumor suppressive effect of microRNA-152 (miR-152). (a–c) A549 or H460 cells were transfected with miR-152 or control mimic (control) with or without an FGF2-overexpression plasmid. Proliferation (CCK-8 assay) (a), in vitro migration (b) and in vitro invasion (c) were evaluated as described in the Materials and methods. (d) FGF2 expression in A549 or H460 cells transfected with an FGF2-overexpression plasmid or the empty vector (control) was measured by quantitative real-time PCR (qRT-PCR). *P<0.05, **P<0.01 vs control. #P<0.05, ##P<0.01 vs miR-152 group.
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fig6: Fibroblast growth factor 2 (FGF2) overexpression partially attenuated the tumor suppressive effect of microRNA-152 (miR-152). (a–c) A549 or H460 cells were transfected with miR-152 or control mimic (control) with or without an FGF2-overexpression plasmid. Proliferation (CCK-8 assay) (a), in vitro migration (b) and in vitro invasion (c) were evaluated as described in the Materials and methods. (d) FGF2 expression in A549 or H460 cells transfected with an FGF2-overexpression plasmid or the empty vector (control) was measured by quantitative real-time PCR (qRT-PCR). *P<0.05, **P<0.01 vs control. #P<0.05, ##P<0.01 vs miR-152 group.

Mentions: We further investigated whether FGF2 overexpression could attenuate the tumor suppressive effects of miR-152. Indeed, CCK-8 assays (Figure 6a) as well as in vitro migration and invasion assays (Figures 6b and c) revealed that overexpression of FGF2 significantly reversed the tumor suppressive effects of miR-152 on A549 cells. These effects were validated using another NSCLC cell line, H460. Increased levels of FGF2 mRNA after cells were transfected with an FGF2 plasmid were confirmed by qRT-PCR (Figure 6d). These data indicate that restoration of FGF2 significantly attenuated the tumor suppressive effect of miR-152.


MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2.

Cheng Z, Ma R, Tan W, Zhang L - Exp. Mol. Med. (2014)

Fibroblast growth factor 2 (FGF2) overexpression partially attenuated the tumor suppressive effect of microRNA-152 (miR-152). (a–c) A549 or H460 cells were transfected with miR-152 or control mimic (control) with or without an FGF2-overexpression plasmid. Proliferation (CCK-8 assay) (a), in vitro migration (b) and in vitro invasion (c) were evaluated as described in the Materials and methods. (d) FGF2 expression in A549 or H460 cells transfected with an FGF2-overexpression plasmid or the empty vector (control) was measured by quantitative real-time PCR (qRT-PCR). *P<0.05, **P<0.01 vs control. #P<0.05, ##P<0.01 vs miR-152 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150934&req=5

fig6: Fibroblast growth factor 2 (FGF2) overexpression partially attenuated the tumor suppressive effect of microRNA-152 (miR-152). (a–c) A549 or H460 cells were transfected with miR-152 or control mimic (control) with or without an FGF2-overexpression plasmid. Proliferation (CCK-8 assay) (a), in vitro migration (b) and in vitro invasion (c) were evaluated as described in the Materials and methods. (d) FGF2 expression in A549 or H460 cells transfected with an FGF2-overexpression plasmid or the empty vector (control) was measured by quantitative real-time PCR (qRT-PCR). *P<0.05, **P<0.01 vs control. #P<0.05, ##P<0.01 vs miR-152 group.
Mentions: We further investigated whether FGF2 overexpression could attenuate the tumor suppressive effects of miR-152. Indeed, CCK-8 assays (Figure 6a) as well as in vitro migration and invasion assays (Figures 6b and c) revealed that overexpression of FGF2 significantly reversed the tumor suppressive effects of miR-152 on A549 cells. These effects were validated using another NSCLC cell line, H460. Increased levels of FGF2 mRNA after cells were transfected with an FGF2 plasmid were confirmed by qRT-PCR (Figure 6d). These data indicate that restoration of FGF2 significantly attenuated the tumor suppressive effect of miR-152.

Bottom Line: MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells.Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion.FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, ZhongNan Hospital of WuHan University, WuHan, China.

ABSTRACT
MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells. Here, we showed that miR-152 was downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion. Fibroblast growth factor 2 (FGF2) was confirmed as a direct target of miR-152. FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152. Furthermore, the presence of miR-152 was inversely correlated with FGF2 in NSCLC tissues. Overall, this study demonstrated that miR-152 suppressed the proliferation and invasion of NSCLC cells by downregulating FGF2. These findings provide novel insights with potential therapeutic applications for the treatment of NSCLC.

Show MeSH
Related in: MedlinePlus