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MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2.

Cheng Z, Ma R, Tan W, Zhang L - Exp. Mol. Med. (2014)

Bottom Line: MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells.Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion.FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, ZhongNan Hospital of WuHan University, WuHan, China.

ABSTRACT
MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells. Here, we showed that miR-152 was downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion. Fibroblast growth factor 2 (FGF2) was confirmed as a direct target of miR-152. FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152. Furthermore, the presence of miR-152 was inversely correlated with FGF2 in NSCLC tissues. Overall, this study demonstrated that miR-152 suppressed the proliferation and invasion of NSCLC cells by downregulating FGF2. These findings provide novel insights with potential therapeutic applications for the treatment of NSCLC.

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Related in: MedlinePlus

MicroRNA-152 (miR-152) suppressed the migration and invasion of non-small-cell lung cancer (NSCLC) cells. (a) A549 cells were transfected with miR-152 or control mimics, and in vitro migration was assessed. (b) In vitro invasion assay. *P<0.05, **P<0.01 vs control.
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fig3: MicroRNA-152 (miR-152) suppressed the migration and invasion of non-small-cell lung cancer (NSCLC) cells. (a) A549 cells were transfected with miR-152 or control mimics, and in vitro migration was assessed. (b) In vitro invasion assay. *P<0.05, **P<0.01 vs control.

Mentions: Migration and invasion are fundamental functions underlying several cellular processes, including angiogenesis, immune response and metastasis of cancer cells. Here, Transwell chambers were used to measure the number of cells that traversed a porous membrane or moved through an extracellular matrix. These assays revealed that overexpression of miR-152 significantly decreased migration (Figure 3a) and invasion (Figure 3b) of A549 cells, suggesting that miR-152 suppressed the motility of NSCLC cells.


MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2.

Cheng Z, Ma R, Tan W, Zhang L - Exp. Mol. Med. (2014)

MicroRNA-152 (miR-152) suppressed the migration and invasion of non-small-cell lung cancer (NSCLC) cells. (a) A549 cells were transfected with miR-152 or control mimics, and in vitro migration was assessed. (b) In vitro invasion assay. *P<0.05, **P<0.01 vs control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150934&req=5

fig3: MicroRNA-152 (miR-152) suppressed the migration and invasion of non-small-cell lung cancer (NSCLC) cells. (a) A549 cells were transfected with miR-152 or control mimics, and in vitro migration was assessed. (b) In vitro invasion assay. *P<0.05, **P<0.01 vs control.
Mentions: Migration and invasion are fundamental functions underlying several cellular processes, including angiogenesis, immune response and metastasis of cancer cells. Here, Transwell chambers were used to measure the number of cells that traversed a porous membrane or moved through an extracellular matrix. These assays revealed that overexpression of miR-152 significantly decreased migration (Figure 3a) and invasion (Figure 3b) of A549 cells, suggesting that miR-152 suppressed the motility of NSCLC cells.

Bottom Line: MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells.Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion.FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, ZhongNan Hospital of WuHan University, WuHan, China.

ABSTRACT
MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells. Here, we showed that miR-152 was downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion. Fibroblast growth factor 2 (FGF2) was confirmed as a direct target of miR-152. FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152. Furthermore, the presence of miR-152 was inversely correlated with FGF2 in NSCLC tissues. Overall, this study demonstrated that miR-152 suppressed the proliferation and invasion of NSCLC cells by downregulating FGF2. These findings provide novel insights with potential therapeutic applications for the treatment of NSCLC.

Show MeSH
Related in: MedlinePlus