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MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2.

Cheng Z, Ma R, Tan W, Zhang L - Exp. Mol. Med. (2014)

Bottom Line: MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells.Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion.FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, ZhongNan Hospital of WuHan University, WuHan, China.

ABSTRACT
MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells. Here, we showed that miR-152 was downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion. Fibroblast growth factor 2 (FGF2) was confirmed as a direct target of miR-152. FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152. Furthermore, the presence of miR-152 was inversely correlated with FGF2 in NSCLC tissues. Overall, this study demonstrated that miR-152 suppressed the proliferation and invasion of NSCLC cells by downregulating FGF2. These findings provide novel insights with potential therapeutic applications for the treatment of NSCLC.

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Related in: MedlinePlus

MicroRNA-152 (miR-152) was decreased in non-small-cell lung cancer (NSCLC) tissues and cell lines. (a) The expression levels of miR-152 in 30 pairs of NSCLC tissues and their matched normal tissues were measured by quantitative real-time PCR (qRT-PCR). U6 was used as an internal control. (b) The expression levels of miR-152 in a normal lung epithelial cell line (16HBE) and four NSCLC cell lines (A549, SK-MES-1, H460 and H520). *P<0.05, **P<0.01 vs control.
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fig1: MicroRNA-152 (miR-152) was decreased in non-small-cell lung cancer (NSCLC) tissues and cell lines. (a) The expression levels of miR-152 in 30 pairs of NSCLC tissues and their matched normal tissues were measured by quantitative real-time PCR (qRT-PCR). U6 was used as an internal control. (b) The expression levels of miR-152 in a normal lung epithelial cell line (16HBE) and four NSCLC cell lines (A549, SK-MES-1, H460 and H520). *P<0.05, **P<0.01 vs control.

Mentions: The expression of miR-152 in 30 pairs of NSCLC tissues and their matched normal tissues was measured using qRT-PCR. The results showed that miR-152 expression was significantly downregulated in NSCLC tissues compared with matched controls (Figure 1a). In addition, the expression of miR-152 in four NSCLC cell lines was determined. We found that the relative expression of miR-152 in these NSCLC cells was strikingly decreased compared with that of the normal lung bronchus epithelial cell line 16HBE (Figure 1b). These results suggest that downregulation of miR-152 may contribute to the progression of NSCLC.


MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2.

Cheng Z, Ma R, Tan W, Zhang L - Exp. Mol. Med. (2014)

MicroRNA-152 (miR-152) was decreased in non-small-cell lung cancer (NSCLC) tissues and cell lines. (a) The expression levels of miR-152 in 30 pairs of NSCLC tissues and their matched normal tissues were measured by quantitative real-time PCR (qRT-PCR). U6 was used as an internal control. (b) The expression levels of miR-152 in a normal lung epithelial cell line (16HBE) and four NSCLC cell lines (A549, SK-MES-1, H460 and H520). *P<0.05, **P<0.01 vs control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150934&req=5

fig1: MicroRNA-152 (miR-152) was decreased in non-small-cell lung cancer (NSCLC) tissues and cell lines. (a) The expression levels of miR-152 in 30 pairs of NSCLC tissues and their matched normal tissues were measured by quantitative real-time PCR (qRT-PCR). U6 was used as an internal control. (b) The expression levels of miR-152 in a normal lung epithelial cell line (16HBE) and four NSCLC cell lines (A549, SK-MES-1, H460 and H520). *P<0.05, **P<0.01 vs control.
Mentions: The expression of miR-152 in 30 pairs of NSCLC tissues and their matched normal tissues was measured using qRT-PCR. The results showed that miR-152 expression was significantly downregulated in NSCLC tissues compared with matched controls (Figure 1a). In addition, the expression of miR-152 in four NSCLC cell lines was determined. We found that the relative expression of miR-152 in these NSCLC cells was strikingly decreased compared with that of the normal lung bronchus epithelial cell line 16HBE (Figure 1b). These results suggest that downregulation of miR-152 may contribute to the progression of NSCLC.

Bottom Line: MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells.Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion.FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, ZhongNan Hospital of WuHan University, WuHan, China.

ABSTRACT
MicroRNAs (miRNAs) regulate the proliferation and metastasis of cancer cells. Here, we showed that miR-152 was downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. Overexpression of miR-152 suppressed cell proliferation and colony formation and also limited migration and invasion. Fibroblast growth factor 2 (FGF2) was confirmed as a direct target of miR-152. FGF2 knockdown suppressed cell proliferation, colony formation, migration and invasion, whereas FGF2 overexpression partially reversed the suppressive effect of miR-152. Furthermore, the presence of miR-152 was inversely correlated with FGF2 in NSCLC tissues. Overall, this study demonstrated that miR-152 suppressed the proliferation and invasion of NSCLC cells by downregulating FGF2. These findings provide novel insights with potential therapeutic applications for the treatment of NSCLC.

Show MeSH
Related in: MedlinePlus