Limits...
Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue.

Wang Y, Liao L - BMC Urol (2014)

Bottom Line: The results were quantified as optical density (OD) values.In the in vitro detrusor strip study, there were no significant differences in autorhythmicity and contractility between regenerated and normal tissues in group C (p > 0.05).SIS seems to be a viable material in the reconstruction of the rabbit urinary bladder.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Urology, China Rehabilitation Research Center, 10 Jiaomen Beilu, Beijing 100068, Fentai District, China. lmliao@263.net.

ABSTRACT

Background: Intestinal bladder augmentation has more disadvantages. One of the most promising alternative methods is tissue engineering in combination with surgical construction. Small intestine submucosa (SIS) is commonly used materials in tissue engineer. The aim of this study is determine the histologic and functional characteristics of SIS as bladder wall replacement in a rabbit augmentation model.

Methods: 18 New Zealand adult male rabbits, weight 2.5 ± 0.5Kg, were used in this study. The rabbits were divided into 3 groups of 6 based on the number of days post-operative (A, 4 weeks; B, 12 weeks; C, 24 weeks). All of the animals underwent urodynamic testing under anesthesia before cystoplasty with SIS patch. The cystometrograms were repeated 4, 12, and 24 weeks after surgery with the same method. SIS-regenerated bladder strips (10 × 3 × 3 mm) and normal bladder strips (10 × 3 × 3 mm) from the same bladder were obtained at 4, 12, and 24 weeks for in vitro detrusor strip study. The frequency and amplitude of the strip over 15 min was recorded. The regenerated tissue and normal tissue underwent histologic and immunocytochemical analysis. The results were quantified as optical density (OD) values.

Results: Histologically, the SIS-regenerated bladders of group C (24 weeks post-operation) resembled normal bladder in that all 3 layers (mucosa with submucosa, smooth muscle, and serosa) were present. In the in vitro detrusor strip study, there were no significant differences in autorhythmicity and contractility between regenerated and normal tissues in group C (p > 0.05). Immunohistochemical analysis indicated that the quantity of A-actin grew to a normal level. Urodynamic testing showed that compliance remained stable in all groups post-operatively, and the volume increased 24 weeks post-operatively.

Conclusion: Regenerated tissue has similar histologic and functional characteristics. SIS seems to be a viable material in the reconstruction of the rabbit urinary bladder.

Show MeSH

Related in: MedlinePlus

Compare of OD value. The OD value of regenerated tissue was 0.067 ± 0.022 in group A, 0.082 ± 0.017 in group B, and 0.148 ± 0.099 in group C. Compared with normal bladder (0.140 ± 0.021 in group A, 0.130 ± 0.020 in group B, 0.152 ± 0.017 in group C), groups A and B were significantly different with normal bladder (p < 0.05), while there was no significant difference in group C and normal bladder (p > 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4150863&req=5

Figure 2: Compare of OD value. The OD value of regenerated tissue was 0.067 ± 0.022 in group A, 0.082 ± 0.017 in group B, and 0.148 ± 0.099 in group C. Compared with normal bladder (0.140 ± 0.021 in group A, 0.130 ± 0.020 in group B, 0.152 ± 0.017 in group C), groups A and B were significantly different with normal bladder (p < 0.05), while there was no significant difference in group C and normal bladder (p > 0.05).

Mentions: All rabbits were euthanized. The outer and inner surfaces of the bladder, perivesical fat, kidneys, and ureters were evaluated macroscopically. The kidneys and ureters were grossly normal without evidence of hydronephrosis. There were no diverticula in any group. There was no extravasation or contractions in the graft regions. The material was degraded in groups B and C, but not degraded on the inner surface in group A. Regenerated tissue covered the outer surface of the region of the graft, which was indistinguishable from the normal host bladder at the outer and inner surfaces in group C (Figure 2b).


Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue.

Wang Y, Liao L - BMC Urol (2014)

Compare of OD value. The OD value of regenerated tissue was 0.067 ± 0.022 in group A, 0.082 ± 0.017 in group B, and 0.148 ± 0.099 in group C. Compared with normal bladder (0.140 ± 0.021 in group A, 0.130 ± 0.020 in group B, 0.152 ± 0.017 in group C), groups A and B were significantly different with normal bladder (p < 0.05), while there was no significant difference in group C and normal bladder (p > 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4150863&req=5

Figure 2: Compare of OD value. The OD value of regenerated tissue was 0.067 ± 0.022 in group A, 0.082 ± 0.017 in group B, and 0.148 ± 0.099 in group C. Compared with normal bladder (0.140 ± 0.021 in group A, 0.130 ± 0.020 in group B, 0.152 ± 0.017 in group C), groups A and B were significantly different with normal bladder (p < 0.05), while there was no significant difference in group C and normal bladder (p > 0.05).
Mentions: All rabbits were euthanized. The outer and inner surfaces of the bladder, perivesical fat, kidneys, and ureters were evaluated macroscopically. The kidneys and ureters were grossly normal without evidence of hydronephrosis. There were no diverticula in any group. There was no extravasation or contractions in the graft regions. The material was degraded in groups B and C, but not degraded on the inner surface in group A. Regenerated tissue covered the outer surface of the region of the graft, which was indistinguishable from the normal host bladder at the outer and inner surfaces in group C (Figure 2b).

Bottom Line: The results were quantified as optical density (OD) values.In the in vitro detrusor strip study, there were no significant differences in autorhythmicity and contractility between regenerated and normal tissues in group C (p > 0.05).SIS seems to be a viable material in the reconstruction of the rabbit urinary bladder.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Urology, China Rehabilitation Research Center, 10 Jiaomen Beilu, Beijing 100068, Fentai District, China. lmliao@263.net.

ABSTRACT

Background: Intestinal bladder augmentation has more disadvantages. One of the most promising alternative methods is tissue engineering in combination with surgical construction. Small intestine submucosa (SIS) is commonly used materials in tissue engineer. The aim of this study is determine the histologic and functional characteristics of SIS as bladder wall replacement in a rabbit augmentation model.

Methods: 18 New Zealand adult male rabbits, weight 2.5 ± 0.5Kg, were used in this study. The rabbits were divided into 3 groups of 6 based on the number of days post-operative (A, 4 weeks; B, 12 weeks; C, 24 weeks). All of the animals underwent urodynamic testing under anesthesia before cystoplasty with SIS patch. The cystometrograms were repeated 4, 12, and 24 weeks after surgery with the same method. SIS-regenerated bladder strips (10 × 3 × 3 mm) and normal bladder strips (10 × 3 × 3 mm) from the same bladder were obtained at 4, 12, and 24 weeks for in vitro detrusor strip study. The frequency and amplitude of the strip over 15 min was recorded. The regenerated tissue and normal tissue underwent histologic and immunocytochemical analysis. The results were quantified as optical density (OD) values.

Results: Histologically, the SIS-regenerated bladders of group C (24 weeks post-operation) resembled normal bladder in that all 3 layers (mucosa with submucosa, smooth muscle, and serosa) were present. In the in vitro detrusor strip study, there were no significant differences in autorhythmicity and contractility between regenerated and normal tissues in group C (p > 0.05). Immunohistochemical analysis indicated that the quantity of A-actin grew to a normal level. Urodynamic testing showed that compliance remained stable in all groups post-operatively, and the volume increased 24 weeks post-operatively.

Conclusion: Regenerated tissue has similar histologic and functional characteristics. SIS seems to be a viable material in the reconstruction of the rabbit urinary bladder.

Show MeSH
Related in: MedlinePlus