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Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue.

Wang Y, Liao L - BMC Urol (2014)

Bottom Line: The results were quantified as optical density (OD) values.In the in vitro detrusor strip study, there were no significant differences in autorhythmicity and contractility between regenerated and normal tissues in group C (p > 0.05).SIS seems to be a viable material in the reconstruction of the rabbit urinary bladder.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Urology, China Rehabilitation Research Center, 10 Jiaomen Beilu, Beijing 100068, Fentai District, China. lmliao@263.net.

ABSTRACT

Background: Intestinal bladder augmentation has more disadvantages. One of the most promising alternative methods is tissue engineering in combination with surgical construction. Small intestine submucosa (SIS) is commonly used materials in tissue engineer. The aim of this study is determine the histologic and functional characteristics of SIS as bladder wall replacement in a rabbit augmentation model.

Methods: 18 New Zealand adult male rabbits, weight 2.5 ± 0.5Kg, were used in this study. The rabbits were divided into 3 groups of 6 based on the number of days post-operative (A, 4 weeks; B, 12 weeks; C, 24 weeks). All of the animals underwent urodynamic testing under anesthesia before cystoplasty with SIS patch. The cystometrograms were repeated 4, 12, and 24 weeks after surgery with the same method. SIS-regenerated bladder strips (10 × 3 × 3 mm) and normal bladder strips (10 × 3 × 3 mm) from the same bladder were obtained at 4, 12, and 24 weeks for in vitro detrusor strip study. The frequency and amplitude of the strip over 15 min was recorded. The regenerated tissue and normal tissue underwent histologic and immunocytochemical analysis. The results were quantified as optical density (OD) values.

Results: Histologically, the SIS-regenerated bladders of group C (24 weeks post-operation) resembled normal bladder in that all 3 layers (mucosa with submucosa, smooth muscle, and serosa) were present. In the in vitro detrusor strip study, there were no significant differences in autorhythmicity and contractility between regenerated and normal tissues in group C (p > 0.05). Immunohistochemical analysis indicated that the quantity of A-actin grew to a normal level. Urodynamic testing showed that compliance remained stable in all groups post-operatively, and the volume increased 24 weeks post-operatively.

Conclusion: Regenerated tissue has similar histologic and functional characteristics. SIS seems to be a viable material in the reconstruction of the rabbit urinary bladder.

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Related in: MedlinePlus

Macroscopic and HE evaluation. a. The SIS patch (1.0 × 2.0 cm) was already grafted onto the host bladder. b. Regenerated tissue in the arrows 24 weeks post-operation. c. Thin arrow marked the regenerated transitional epithelium in the region of the SIS graft. Coarse arrow marked the infiltrated inflammatory cells (×20). d. Thin arrow marked the regenerated transitional epithelium. Coarse arrow marked the new vessels (×10).
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Figure 1: Macroscopic and HE evaluation. a. The SIS patch (1.0 × 2.0 cm) was already grafted onto the host bladder. b. Regenerated tissue in the arrows 24 weeks post-operation. c. Thin arrow marked the regenerated transitional epithelium in the region of the SIS graft. Coarse arrow marked the infiltrated inflammatory cells (×20). d. Thin arrow marked the regenerated transitional epithelium. Coarse arrow marked the new vessels (×10).

Mentions: After approval of the ethics committee of the China Rehabilitation Research Center, 18 New Zealand adult male rabbits, weight 2.5 ± 0.5 Kg, were used in this study. All the animals were in good health. The rabbits were divided into 3 groups of 6 based on the number of days post-operative (A, 4 weeks; B, 12 weeks; C, 24 weeks). The abdominal regions of the rabbits were shaved after anesthesia with 2% phenobarbital (30 mg/kg). The abdomen was opened through a midline incision and the bladder was exposed. The anterior wall of the bladder was opened longitudinally through a 3-cm incision in the midline of the bladder body. The SIS patch (1.0 × 2.0 cm) was grafted onto the host bladder with a 5/0 vicryl interrupted suture (Figure 1a). Four 5/0 silk marking stitches were placed outside the bladder wall near the corners of the patch. Perivesical fat was fixed over the bladder wall to cover the graft and the abdominal wall was closed anatomically. A single dose of ceftriaxone was administered (500 mg intramuscular). Neither urinary diversion nor urethral catheterization was used. The rabbits were housed and fed in separate cages.


Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue.

Wang Y, Liao L - BMC Urol (2014)

Macroscopic and HE evaluation. a. The SIS patch (1.0 × 2.0 cm) was already grafted onto the host bladder. b. Regenerated tissue in the arrows 24 weeks post-operation. c. Thin arrow marked the regenerated transitional epithelium in the region of the SIS graft. Coarse arrow marked the infiltrated inflammatory cells (×20). d. Thin arrow marked the regenerated transitional epithelium. Coarse arrow marked the new vessels (×10).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4150863&req=5

Figure 1: Macroscopic and HE evaluation. a. The SIS patch (1.0 × 2.0 cm) was already grafted onto the host bladder. b. Regenerated tissue in the arrows 24 weeks post-operation. c. Thin arrow marked the regenerated transitional epithelium in the region of the SIS graft. Coarse arrow marked the infiltrated inflammatory cells (×20). d. Thin arrow marked the regenerated transitional epithelium. Coarse arrow marked the new vessels (×10).
Mentions: After approval of the ethics committee of the China Rehabilitation Research Center, 18 New Zealand adult male rabbits, weight 2.5 ± 0.5 Kg, were used in this study. All the animals were in good health. The rabbits were divided into 3 groups of 6 based on the number of days post-operative (A, 4 weeks; B, 12 weeks; C, 24 weeks). The abdominal regions of the rabbits were shaved after anesthesia with 2% phenobarbital (30 mg/kg). The abdomen was opened through a midline incision and the bladder was exposed. The anterior wall of the bladder was opened longitudinally through a 3-cm incision in the midline of the bladder body. The SIS patch (1.0 × 2.0 cm) was grafted onto the host bladder with a 5/0 vicryl interrupted suture (Figure 1a). Four 5/0 silk marking stitches were placed outside the bladder wall near the corners of the patch. Perivesical fat was fixed over the bladder wall to cover the graft and the abdominal wall was closed anatomically. A single dose of ceftriaxone was administered (500 mg intramuscular). Neither urinary diversion nor urethral catheterization was used. The rabbits were housed and fed in separate cages.

Bottom Line: The results were quantified as optical density (OD) values.In the in vitro detrusor strip study, there were no significant differences in autorhythmicity and contractility between regenerated and normal tissues in group C (p > 0.05).SIS seems to be a viable material in the reconstruction of the rabbit urinary bladder.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Urology, China Rehabilitation Research Center, 10 Jiaomen Beilu, Beijing 100068, Fentai District, China. lmliao@263.net.

ABSTRACT

Background: Intestinal bladder augmentation has more disadvantages. One of the most promising alternative methods is tissue engineering in combination with surgical construction. Small intestine submucosa (SIS) is commonly used materials in tissue engineer. The aim of this study is determine the histologic and functional characteristics of SIS as bladder wall replacement in a rabbit augmentation model.

Methods: 18 New Zealand adult male rabbits, weight 2.5 ± 0.5Kg, were used in this study. The rabbits were divided into 3 groups of 6 based on the number of days post-operative (A, 4 weeks; B, 12 weeks; C, 24 weeks). All of the animals underwent urodynamic testing under anesthesia before cystoplasty with SIS patch. The cystometrograms were repeated 4, 12, and 24 weeks after surgery with the same method. SIS-regenerated bladder strips (10 × 3 × 3 mm) and normal bladder strips (10 × 3 × 3 mm) from the same bladder were obtained at 4, 12, and 24 weeks for in vitro detrusor strip study. The frequency and amplitude of the strip over 15 min was recorded. The regenerated tissue and normal tissue underwent histologic and immunocytochemical analysis. The results were quantified as optical density (OD) values.

Results: Histologically, the SIS-regenerated bladders of group C (24 weeks post-operation) resembled normal bladder in that all 3 layers (mucosa with submucosa, smooth muscle, and serosa) were present. In the in vitro detrusor strip study, there were no significant differences in autorhythmicity and contractility between regenerated and normal tissues in group C (p > 0.05). Immunohistochemical analysis indicated that the quantity of A-actin grew to a normal level. Urodynamic testing showed that compliance remained stable in all groups post-operatively, and the volume increased 24 weeks post-operatively.

Conclusion: Regenerated tissue has similar histologic and functional characteristics. SIS seems to be a viable material in the reconstruction of the rabbit urinary bladder.

Show MeSH
Related in: MedlinePlus