Drosophila COP9 signalosome subunit 7 interacts with multiple genomic loci to regulate development.
Bottom Line: While the function of this complex in ubiquitin-mediated protein degradation is well established, results over the past few years have hinted that the COP9 signalosome may function more broadly in the regulation of gene expression.These results indicate that CSN7, and by extension the entire COP9 signalosome, functions directly in transcriptional control.While the COP9 signalosome protein complex has long been known to regulate protein degradation, here we expand the role of this complex by showing that subunit 7 binds DNA in vitro and functions directly in vivo in transcriptional control of developmentally important pathways that are relevant for human health.
Affiliation: Department of Molecular Biology and Ecology of Plants.Show MeSH
Mentions: The bioinformatic analysis above clearly illustrated that CSN-dependent genes are enriched in the CSN7-DamID dataset. To further connect between the genes bound by CSN7 and the phenotypes described above, we determined if the expression of the genes within the CSN7-DamID loci is also misregulated upon RNAi-mediated reduction of CSN7. We examined by qRT-PCR the mRNA levels of three key developmental genes involved in cell-cycle regulation and wing disc development, CycE, E2f and Scalloped (Sd). mRNA levels were checked in Kc167 and S2 cells where we reduced CSN7 levels via RNAi. As shown in Figure 4A and Supplementary Figure S6, the levels of CycE and E2f were reduced when Csn7 was silenced in both cell types, while Sd was reduced only in Kc167 cells.
Affiliation: Department of Molecular Biology and Ecology of Plants.