Drosophila COP9 signalosome subunit 7 interacts with multiple genomic loci to regulate development.
Bottom Line: While the function of this complex in ubiquitin-mediated protein degradation is well established, results over the past few years have hinted that the COP9 signalosome may function more broadly in the regulation of gene expression.These results indicate that CSN7, and by extension the entire COP9 signalosome, functions directly in transcriptional control.While the COP9 signalosome protein complex has long been known to regulate protein degradation, here we expand the role of this complex by showing that subunit 7 binds DNA in vitro and functions directly in vivo in transcriptional control of developmentally important pathways that are relevant for human health.
Affiliation: Department of Molecular Biology and Ecology of Plants.Show MeSH
Mentions: Next, we examined the role of CSN7 in imaginal disc formation and cell-cycle progression, two of the GO groupings identified as enriched in CSN7-DamID targets. Imaginal wing discs in third instar csn7p larvae are severely reduced in size, which correlates with the enrichment of genes associated with imaginal discs identified by DamID. As the size of the imaginal wing discs from the csn7 p mutant discs precluded further analyses, we specifically targeted CSN7 using UAS-Csn7-RNAi transgene under either Act-Gal4 or En-Gal4. While these wing discs were of normal size, they were developmentally defective as the folds in the wing pouch and hinge were not readily evident (Figure 3A and B).
Affiliation: Department of Molecular Biology and Ecology of Plants.