Human fetal globin gene expression is regulated by LYAR.
Bottom Line: We found that PRMT5 binding on the proximal γ-promoter was LYAR-dependent.We also found that LYAR repressed human fetal globin gene expression in both K562 cells and primary human adult erythroid progenitor cells.Thus, these data indicate that LYAR acts as a novel transcription factor that binds the γ-globin gene, and is essential for silencing the γ-globin gene.
Affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.Show MeSH
Related in: MedlinePlus
Mentions: Human γ-globin gene expression is developmentally regulated, and is gradually silenced after birth. To examine the possibility that LYAR plays a role in this process, western blot analysis was performed using an anti-LYAR antibody in human CB and AE progenitor cells. High levels of LYAR were observed in AE progenitor cells (γ-globin ‘off’ state), whereas barely detectable levels of LYAR were found in CB cells (γ-globin ‘on’ state) (Figure 6A). This is consistent with the putative role of LYAR as a repressor of γ-globin expression.
Affiliation: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.