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Regulation of pri-miRNA processing by the hnRNP-like protein AtGRP7 in Arabidopsis.

Köster T, Meyer K, Weinholdt C, Smith LM, Lummer M, Speth C, Grosse I, Weigel D, Staiger D - Nucleic Acids Res. (2014)

Bottom Line: AtGRP7 overexpression caused a significant reduction in the level of 30 miRNAs and an increase for 14 miRNAs with a minimum log2 fold change of ± 0.5.Mutation of an arginine in the RNA recognition motif abrogated in vivo binding and the effect on miRNA and pri-miRNA levels, indicating that AtGRP7 inhibits processing of these pri-miRNAs by direct binding.Thus, AtGRP7 is an hnRNP-like protein with a role in processing of pri-miRNAs in addition to its role in pre-mRNA splicing.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cell Physiology, Bielefeld University.

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MiR398 levels are reduced in AtGRP7-ox plants. An RNA gel blot of the AtGRP7-ox lines D and G in Col-2, AtGRP7-ox lines in L er and C24 and the corresponding wt plants was hybridised with anti-miR398 (top) and a U6 control (bottom). Fold changes of miR398 normalized to U6 in AtGRP7-ox plants are expressed relative to wt.
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Figure 1: MiR398 levels are reduced in AtGRP7-ox plants. An RNA gel blot of the AtGRP7-ox lines D and G in Col-2, AtGRP7-ox lines in L er and C24 and the corresponding wt plants was hybridised with anti-miR398 (top) and a U6 control (bottom). Fold changes of miR398 normalized to U6 in AtGRP7-ox plants are expressed relative to wt.

Mentions: Genome-wide transcriptome analysis of AtGRP7-ox plants had previously revealed an elevated steady-state abundance of the COPPER ZINC SUPEROXIDE DISMUTASE 2 (CSD2) transcript encoding a superoxide dismutase that uses copper as a metal cofactor (47). CSD2 is a target of the miR398 family, which comprises identical miRNAs encoded by MIR398b and MIR398c on chromosome V, and a miRNA with a 3’ U instead of G encoded by MIR398a on chromosome II (48,49). To test whether the altered CSD2 transcript level in AtGRP7-ox plants reflected an impact of AtGRP7 on miR398, we monitored miR398 levels using low molecular weight northern blot analysis. MiR398 steady-state abundance was reduced in AtGRP7-ox lines in the Col-2, L er and C24 accessions compared to the corresponding wt plants (Figure 1).


Regulation of pri-miRNA processing by the hnRNP-like protein AtGRP7 in Arabidopsis.

Köster T, Meyer K, Weinholdt C, Smith LM, Lummer M, Speth C, Grosse I, Weigel D, Staiger D - Nucleic Acids Res. (2014)

MiR398 levels are reduced in AtGRP7-ox plants. An RNA gel blot of the AtGRP7-ox lines D and G in Col-2, AtGRP7-ox lines in L er and C24 and the corresponding wt plants was hybridised with anti-miR398 (top) and a U6 control (bottom). Fold changes of miR398 normalized to U6 in AtGRP7-ox plants are expressed relative to wt.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150807&req=5

Figure 1: MiR398 levels are reduced in AtGRP7-ox plants. An RNA gel blot of the AtGRP7-ox lines D and G in Col-2, AtGRP7-ox lines in L er and C24 and the corresponding wt plants was hybridised with anti-miR398 (top) and a U6 control (bottom). Fold changes of miR398 normalized to U6 in AtGRP7-ox plants are expressed relative to wt.
Mentions: Genome-wide transcriptome analysis of AtGRP7-ox plants had previously revealed an elevated steady-state abundance of the COPPER ZINC SUPEROXIDE DISMUTASE 2 (CSD2) transcript encoding a superoxide dismutase that uses copper as a metal cofactor (47). CSD2 is a target of the miR398 family, which comprises identical miRNAs encoded by MIR398b and MIR398c on chromosome V, and a miRNA with a 3’ U instead of G encoded by MIR398a on chromosome II (48,49). To test whether the altered CSD2 transcript level in AtGRP7-ox plants reflected an impact of AtGRP7 on miR398, we monitored miR398 levels using low molecular weight northern blot analysis. MiR398 steady-state abundance was reduced in AtGRP7-ox lines in the Col-2, L er and C24 accessions compared to the corresponding wt plants (Figure 1).

Bottom Line: AtGRP7 overexpression caused a significant reduction in the level of 30 miRNAs and an increase for 14 miRNAs with a minimum log2 fold change of ± 0.5.Mutation of an arginine in the RNA recognition motif abrogated in vivo binding and the effect on miRNA and pri-miRNA levels, indicating that AtGRP7 inhibits processing of these pri-miRNAs by direct binding.Thus, AtGRP7 is an hnRNP-like protein with a role in processing of pri-miRNAs in addition to its role in pre-mRNA splicing.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cell Physiology, Bielefeld University.

Show MeSH