Regulation of transcription termination by glucosylated hydroxymethyluracil, base J, in Leishmania major and Trypanosoma brucei.
Bottom Line: Reduction of J in Leishmania tarentolae via growth in BrdU resulted in cell death and indicated a role of J in the regulation of RNAP II termination.Reduction of J in L. major resulted in genome-wide defects in transcription termination at the end of polycistronic gene clusters and the generation of antisense RNAs, without cell death.In contrast, loss of J in T. brucei did not lead to genome-wide termination defects; however, the loss of J at specific sites within polycistronic gene clusters led to altered transcription termination and increased expression of downstream genes.
Affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Davison Life Sciences Building, 120 Green Street, Athens, GA 30602-7229, USA.Show MeSH
Related in: MedlinePlus
Mentions: Bloodstream form T. brucei cell line 221a of strain 427 was cultured in HMI-9 medium as described previously (40). L. major parasites were grown at 26°C in M199 media supplemented with 10% fetal bovine serum (FBS) as described (41). DMOG treatment of cells was performed by supplementing media with 1-mM DMOG for 5 days in T. brucei or at 5-mM for 10 days in L. major (1–5 mM for the DMOG titration experiments shown in Figure 3C). BrdU was supplemented into media at 10-μM or 100-μM for 6 days in L. major.
Affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Davison Life Sciences Building, 120 Green Street, Athens, GA 30602-7229, USA.