Major alteration in coxsackievirus B3 genomic RNA structure distinguishes a virulent strain from an avirulent strain.
Bottom Line: Comparative sequence analysis of 170 closely related enteroviruses revealed that the SLII region lacks conservation.Neither the parent SLII nor the remaining domains of the background 5'UTR were structurally altered by the exchange, supporting an independent mechanism of folding and function.We show that the attenuated 5'UTR lacks structure in the SLII cardiovirulence determinant.
Affiliation: Biology Department, University of Nebraska at Omaha, Omaha, NE 68182, USA.Show MeSH
Related in: MedlinePlus
Mentions: The structure of domains I, II, III, IV, V and VI for both CV-B3/28 and CV-B3/GA were determined with chemical probing analysis. The chemical probing results for domain I (Figure 5A–D) and domain V (Figure 5E–H) are shown in Figure 5 as representatives of the overall comparison. No major structure differences between CV-B3/GA and CV-B3/28 were observed in domains I, III, IV, V or VI. Minor alterations were observed in the structural domains outside of SLII, however these changes were highly localized when compared to the clear differences observed in SLII.
Affiliation: Biology Department, University of Nebraska at Omaha, Omaha, NE 68182, USA.