Major alteration in coxsackievirus B3 genomic RNA structure distinguishes a virulent strain from an avirulent strain.
Bottom Line: Comparative sequence analysis of 170 closely related enteroviruses revealed that the SLII region lacks conservation.Neither the parent SLII nor the remaining domains of the background 5'UTR were structurally altered by the exchange, supporting an independent mechanism of folding and function.We show that the attenuated 5'UTR lacks structure in the SLII cardiovirulence determinant.
Affiliation: Biology Department, University of Nebraska at Omaha, Omaha, NE 68182, USA.Show MeSH
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Mentions: To further explore the 5′UTR RNA structure of virulent and avirulent isolates, SLII (104–184) was exchanged between CV-B3/28 and CV-B3/GA to generate the chimeric constructs 28 SLII GA (CV-B3/28 5′UTR with CV-B3/GA SLII) and GA SLII 28 (CV-B3/GA 5′UTR with CV-B3/28 SLII). As found in the parent molecules, 28 SLII GA was heavily modified in the SLII region (Figure 4A and C) and GA SLII 28 was protected (Figure 4B and D). Thus, probing results for 28 SLII GA matched that of the non-chimeric CV-B3/GA SLII and probing results for GA SLII 28 matched that of the non-chimeric CV-B3/28. The other five 5′UTR structural domains in 28 SLII GA and GA SLII 28 were also analysed with chemical probing analysis and no changes occurred in the structural domains outside of SLII in either chimera (data not shown).
Affiliation: Biology Department, University of Nebraska at Omaha, Omaha, NE 68182, USA.