RNA recognition and self-association of CPEB4 is mediated by its tandem RRM domains.
Bottom Line: Self-association does not affect the proteins' ability to interact with RNA as demonstrated by ion mobility-mass spectrometry.Chemical shift effects measured by NMR of the apo forms of the RRM1-RRM2 samples indicate that the two domains are orientated toward each other.We propose a model of the CPEB4 RRM1-RRM2-CPE complex that illustrates the experimental data.
Affiliation: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10, Barcelona 08028, Spain.Show MeSH
Related in: MedlinePlus
Mentions: In order to characterize the interaction of the RRM domains with short RNAs containing the CPE motifs, we have used several complementary techniques that allow us to detect and quantify the interactions. For this purpose, we performed EMSA as well as ITC binding assays using both RRM1 and RRM1–RRM2 and different RNA fragments. The EMSA experiments showed that RRM1–RRM2 tandem domain has a higher affinity for the CPE RNA when compared to the RRM1 alone (Figure 4A).
Affiliation: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac 10, Barcelona 08028, Spain.