Redundancy of primary RNA-binding functions of the bacterial transcription terminator Rho.
Bottom Line: The bacterial transcription terminator, Rho, terminates transcription at half of the operons.These terminators function optimally only through a NusG-assisted recruitment and activation of Rho.Our data also indicate that at these terminators, Rho-EC-bound NusG interaction facilitates the isomerization of Rho into a translocase-competent form by stabilizing the interactions of mRNA with the secondary RNA binding site, thereby overcoming the defects of the primary RNA binding functions.
Affiliation: Laboratory of Transcription, Center for DNA Fingerprinting and Diagnostics, Tuljaguda Complex, 4-1-714 Mozamjahi Road, Nampally, Hyderabad 500001, India.Show MeSH
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Mentions: We performed microarray analyses of MG1655Δrho strain expressing WT and mutant Rho genes from a low copy pCL1920 plasmid and plotted the fold-change in gene expression of different mutants relative to that of the WT (signal intensity with respect to the WT; Figures 1E–H and 2A, Supplementary Figure S5A and S5B). For SBS mutants, MG1655rac− strains (devoid of rac prophage) were used. We observed the following.
Affiliation: Laboratory of Transcription, Center for DNA Fingerprinting and Diagnostics, Tuljaguda Complex, 4-1-714 Mozamjahi Road, Nampally, Hyderabad 500001, India.