The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription.
Bottom Line: In this work, we present evidence of Bud27 modulating RNA pol II transcription elongation.We also reveal that Bud27 associates in vivo with the Sth1 component of the chromatin remodeling complex RSC and mediates its association with RNA pol II.Our data suggest that Bud27, in addition of contributing to Rpb5 folding within the RNA polymerases, also participates in the correct assembly of other chromatin-associated protein complexes, such as RSC, thereby modulating their activity.
Affiliation: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071, Jaén, Spain.Show MeSH
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Mentions: Finally, to further investigate the contribution of Bud27 in transcription elongation, we next analysed, in a bud27Δ mutant strain, the association of the Rpb1 subunit of the RNA pol II with the long transcription unit of the FMP27/YLR454w gene, expressed under the control of GAL1 promoter (31) in a bud27Δ mutant strain. Similarly to what occurs with the previously constitutive genes tested (see Figure 2A), RNA pol II association decreased through the entire ORF of FMP27/YLR454w (Figure 4A). However, we found no significant difference in the profile of the RNA pol II occupancy in the gene when values were normalized to the 5′ amplicon (Figure 4B). This suggests that Bud27 does not influence RNA polymerase II processivity, analysed as the ability of elongating RNA pol II to travel the entire length of the gene (31).
Affiliation: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071, Jaén, Spain.