The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription.
Bottom Line: In this work, we present evidence of Bud27 modulating RNA pol II transcription elongation.We also reveal that Bud27 associates in vivo with the Sth1 component of the chromatin remodeling complex RSC and mediates its association with RNA pol II.Our data suggest that Bud27, in addition of contributing to Rpb5 folding within the RNA polymerases, also participates in the correct assembly of other chromatin-associated protein complexes, such as RSC, thereby modulating their activity.
Affiliation: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071, Jaén, Spain.Show MeSH
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Mentions: Previously, we have proposed a role for Rpb5 in the transition from transcription initiation to elongation (25). If Bud27 influences RNA pol II-dependent transcription in an Rpb5-mediated manner, we should expect transcription elongation defects in bud27Δ cells. To address this question, we first investigated whether Bud27 is able to influence transcription elongation. For this, we used the length-dependent mRNA accumulation (GLAM) assay, commonly employed to detect defects in transcription elongation (22). As shown in Figure 3A, the GLAM ratio for bud27Δ mutant was significantly reduced compared to that observed for the wild-type strain. Consistently, we found that the growth of bud27Δ cells was impaired in the presence of 6-azauracil (6AU) and MPA, two Nucleotide Triphosphate (NTP)-depleting drugs used to detect S. cerevisiae strains defective for transcription elongation (27,28) (Figure 3B). These results strongly suggest a transcription elongation defect in the bud27 strain.
Affiliation: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071, Jaén, Spain.