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New insights into the incorporation of natural suppressor tRNAs at stop codons in Saccharomyces cerevisiae.

Blanchet S, Cornu D, Argentini M, Namy O - Nucleic Acids Res. (2014)

Bottom Line: We found that glutamine, tyrosine and lysine were inserted at UAA and UAG codons, whereas tryptophan, cysteine and arginine were inserted at UGA codon.We also found that two different glutamine tRNA(Gln) were used to insert glutamine at UAA and UAG codons.This work constitutes the first systematic analysis of the amino acids incorporated at stop codons, providing important new insights into the decoding rules used by the ribosome to read the genetic code.

View Article: PubMed Central - PubMed

Affiliation: Institut de Génétique et Microbiologie, Université Paris-Sud, UMR8621, 91400 Orsay, France.

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Proposed rules for stop codon decoding. This figure shows matching between potential suppressor tRNAs and stop codons. For each tRNA, the number of gene copies is indicated in brackets. The non-canonical base pairings appear in color green when they are accepted, red when they are not. Panel A corresponds to glutamine tRNAs, panel B to glutamate and lysine tRNAs, panel C to tyrosine, cysteine and tryptophan tRNAs. S represents a uridine modified with mcm5S2 and Ψ represents a uridine converted into a pseudouridine.
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Figure 5: Proposed rules for stop codon decoding. This figure shows matching between potential suppressor tRNAs and stop codons. For each tRNA, the number of gene copies is indicated in brackets. The non-canonical base pairings appear in color green when they are accepted, red when they are not. Panel A corresponds to glutamine tRNAs, panel B to glutamate and lysine tRNAs, panel C to tyrosine, cysteine and tryptophan tRNAs. S represents a uridine modified with mcm5S2 and Ψ represents a uridine converted into a pseudouridine.

Mentions: Thus, the major tRNAGln with the 3′GUU5′ anticodon is responsible for the insertion of glutamine at UAA codons, whereas the minor tRNAGln with the 3′GUC5′ anticodon is principally responsible for the insertion of glutamine at UAG codons. These results are consistent with the possibilities for base pairing between these anticodons and the two stop codons (Figure 5A).


New insights into the incorporation of natural suppressor tRNAs at stop codons in Saccharomyces cerevisiae.

Blanchet S, Cornu D, Argentini M, Namy O - Nucleic Acids Res. (2014)

Proposed rules for stop codon decoding. This figure shows matching between potential suppressor tRNAs and stop codons. For each tRNA, the number of gene copies is indicated in brackets. The non-canonical base pairings appear in color green when they are accepted, red when they are not. Panel A corresponds to glutamine tRNAs, panel B to glutamate and lysine tRNAs, panel C to tyrosine, cysteine and tryptophan tRNAs. S represents a uridine modified with mcm5S2 and Ψ represents a uridine converted into a pseudouridine.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150775&req=5

Figure 5: Proposed rules for stop codon decoding. This figure shows matching between potential suppressor tRNAs and stop codons. For each tRNA, the number of gene copies is indicated in brackets. The non-canonical base pairings appear in color green when they are accepted, red when they are not. Panel A corresponds to glutamine tRNAs, panel B to glutamate and lysine tRNAs, panel C to tyrosine, cysteine and tryptophan tRNAs. S represents a uridine modified with mcm5S2 and Ψ represents a uridine converted into a pseudouridine.
Mentions: Thus, the major tRNAGln with the 3′GUU5′ anticodon is responsible for the insertion of glutamine at UAA codons, whereas the minor tRNAGln with the 3′GUC5′ anticodon is principally responsible for the insertion of glutamine at UAG codons. These results are consistent with the possibilities for base pairing between these anticodons and the two stop codons (Figure 5A).

Bottom Line: We found that glutamine, tyrosine and lysine were inserted at UAA and UAG codons, whereas tryptophan, cysteine and arginine were inserted at UGA codon.We also found that two different glutamine tRNA(Gln) were used to insert glutamine at UAA and UAG codons.This work constitutes the first systematic analysis of the amino acids incorporated at stop codons, providing important new insights into the decoding rules used by the ribosome to read the genetic code.

View Article: PubMed Central - PubMed

Affiliation: Institut de Génétique et Microbiologie, Université Paris-Sud, UMR8621, 91400 Orsay, France.

Show MeSH