Genome-wide mapping of unexplored essential regions in the Saccharomyces cerevisiae genome: evidence for hidden synthetic lethal combinations in a genetic interaction network.
Bottom Line: Fifty-six of the 67 regions were found to be essential for cell growth, and 49 of these carried co-lethal gene pair(s) that were not previously been detected by synthetic genetic array analysis.This result implies that regions harboring only non-essential genes contain unidentified synthetic lethal combinations at an unexpectedly high frequency, revealing a novel landscape of genetic interactions in the S. cerevisiae genome.Furthermore, this study indicates that segmental deletion might be exploited for not only revealing genome function but also breeding stress-tolerant strains.
Affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.Show MeSH
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Mentions: To present the results in a comprehensive way, we included 1 confirmed essential region (13) and 14 confirmed non-essential regions (13,14,17,23) in the genome-wide mapping and screening. We also included three regions that were confirmed to be deletable during a prior pilot study. Karyotype analysis and phenotypic characteristics of these 18 derivatives are not shown here. Across the whole S. cerevisiae genome, 110 regions varying in content from 9 non-essential genes in the smallest region to 47 non-essential genes in the largest region and an average of 20 non-essential genes were identified (Figure 1 and Table 2).
Affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.