Enhancing immunomodulation on innate immunity by shape transition among RNA triangle, square and pentagon nanovehicles.
Bottom Line: Changing one RNA strand in polygons automatically induced the stretching of the interior angle from 60° to 90° or 108°, resulting in self-assembly of elegant RNA triangles, squares and pentagons.The degree of immunostimulation greatly depended on the size, shape and number of the payload per nanoparticles.Stronger immune response was observed when the number of adjuvants per polygon was increased, demonstrating the advantage of shape transition from triangle to pentagon.
Affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Markey Cancer Center, Nanobiotechnology Center, University of Kentucky, Lexington, KY 40536, USA.Show MeSH
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Mentions: To further evaluate the size and shape of the resulting RNA assemblies, structural characterization of each polygon was conducted by AFM. AFM images of the pRNA 3WJ based polygons revealed that the shapes of resulting polygons were similar to their predicted, theoretical 3D models (Figure 3A). The estimated average dimensions were found to be 13 ± 1.1, 14 ± 1.8 and 17 ± 1.6 nm for triangles, squares and pentagons, respectively. These values do not reflect the true sizes of the RNA polygons due to the AFM tip convolution, but rather demonstrate that the average size of the nanoparticles increases from triangle to pentagon. In addition, the central cavity of each RNA shape is visible, and the size of the cavity gradually increases with the number of polygon vertices. The measured heights for all nanoparticles was found to be ∼2 nm, in agreement with previously reported heights of nucleic acid double helices (26,53).
Affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Markey Cancer Center, Nanobiotechnology Center, University of Kentucky, Lexington, KY 40536, USA.