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Enhancing immunomodulation on innate immunity by shape transition among RNA triangle, square and pentagon nanovehicles.

Khisamutdinov EF, Li H, Jasinski DL, Chen J, Fu J, Guo P - Nucleic Acids Res. (2014)

Bottom Line: Changing one RNA strand in polygons automatically induced the stretching of the interior angle from 60° to 90° or 108°, resulting in self-assembly of elegant RNA triangles, squares and pentagons.The degree of immunostimulation greatly depended on the size, shape and number of the payload per nanoparticles.Stronger immune response was observed when the number of adjuvants per polygon was increased, demonstrating the advantage of shape transition from triangle to pentagon.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Markey Cancer Center, Nanobiotechnology Center, University of Kentucky, Lexington, KY 40536, USA.

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Design of Polygons and assembly properties. (A) 3D modeled structures of polygons with 3WJ motif located at vertices, inner angle corresponds to ∠AOB. (B) The increasing length of the internal strand (green) stretches the 3WJ ∠AOB at which the nanoparticles assemble, along with increasing number of external ‘short’ strands. (C) Assembly properties of polygons evaluated on 7% native PAGEs. Asterisks ‘*’ indicate the Cy5 labeled strands utilized on each type of polygon assembly.
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Figure 2: Design of Polygons and assembly properties. (A) 3D modeled structures of polygons with 3WJ motif located at vertices, inner angle corresponds to ∠AOB. (B) The increasing length of the internal strand (green) stretches the 3WJ ∠AOB at which the nanoparticles assemble, along with increasing number of external ‘short’ strands. (C) Assembly properties of polygons evaluated on 7% native PAGEs. Asterisks ‘*’ indicate the Cy5 labeled strands utilized on each type of polygon assembly.

Mentions: The structural features of the recently discovered ultrastable pRNA 3WJ module from the bacteriophage Phi29 DNA packaging motor were utilized for in silico design of the RNA triangle, square and pentagon 2D polygons. During the computer modeling we used the particular angle of the 3WJ formed by H1 and H2 as an inner angle of the polygons as we hypothesized that the angle could be stretched to a more open conformation. Throughout this report the intra-helical angle between H1 and H2 is denoted as ∠AOB, as shown in figure 1. Each RNA model contained a pRNA 3WJ motif at each vertex, and the inner angles correspond to ∠AOB. The resulting 3D models exhibited flat conformations, as expected from the plane geometry of the 3WJ motif (51) (Figure 2A).


Enhancing immunomodulation on innate immunity by shape transition among RNA triangle, square and pentagon nanovehicles.

Khisamutdinov EF, Li H, Jasinski DL, Chen J, Fu J, Guo P - Nucleic Acids Res. (2014)

Design of Polygons and assembly properties. (A) 3D modeled structures of polygons with 3WJ motif located at vertices, inner angle corresponds to ∠AOB. (B) The increasing length of the internal strand (green) stretches the 3WJ ∠AOB at which the nanoparticles assemble, along with increasing number of external ‘short’ strands. (C) Assembly properties of polygons evaluated on 7% native PAGEs. Asterisks ‘*’ indicate the Cy5 labeled strands utilized on each type of polygon assembly.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4150753&req=5

Figure 2: Design of Polygons and assembly properties. (A) 3D modeled structures of polygons with 3WJ motif located at vertices, inner angle corresponds to ∠AOB. (B) The increasing length of the internal strand (green) stretches the 3WJ ∠AOB at which the nanoparticles assemble, along with increasing number of external ‘short’ strands. (C) Assembly properties of polygons evaluated on 7% native PAGEs. Asterisks ‘*’ indicate the Cy5 labeled strands utilized on each type of polygon assembly.
Mentions: The structural features of the recently discovered ultrastable pRNA 3WJ module from the bacteriophage Phi29 DNA packaging motor were utilized for in silico design of the RNA triangle, square and pentagon 2D polygons. During the computer modeling we used the particular angle of the 3WJ formed by H1 and H2 as an inner angle of the polygons as we hypothesized that the angle could be stretched to a more open conformation. Throughout this report the intra-helical angle between H1 and H2 is denoted as ∠AOB, as shown in figure 1. Each RNA model contained a pRNA 3WJ motif at each vertex, and the inner angles correspond to ∠AOB. The resulting 3D models exhibited flat conformations, as expected from the plane geometry of the 3WJ motif (51) (Figure 2A).

Bottom Line: Changing one RNA strand in polygons automatically induced the stretching of the interior angle from 60° to 90° or 108°, resulting in self-assembly of elegant RNA triangles, squares and pentagons.The degree of immunostimulation greatly depended on the size, shape and number of the payload per nanoparticles.Stronger immune response was observed when the number of adjuvants per polygon was increased, demonstrating the advantage of shape transition from triangle to pentagon.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Markey Cancer Center, Nanobiotechnology Center, University of Kentucky, Lexington, KY 40536, USA.

Show MeSH
Related in: MedlinePlus