Enhancing immunomodulation on innate immunity by shape transition among RNA triangle, square and pentagon nanovehicles.
Bottom Line: Changing one RNA strand in polygons automatically induced the stretching of the interior angle from 60° to 90° or 108°, resulting in self-assembly of elegant RNA triangles, squares and pentagons.The degree of immunostimulation greatly depended on the size, shape and number of the payload per nanoparticles.Stronger immune response was observed when the number of adjuvants per polygon was increased, demonstrating the advantage of shape transition from triangle to pentagon.
Affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Markey Cancer Center, Nanobiotechnology Center, University of Kentucky, Lexington, KY 40536, USA.Show MeSH
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Mentions: In this report, we introduce a conceptual approach to the rational design of stable RNA architectures by stretching the 60° AOB angle (∠) (Figure 1) of the thermodynamically stable pRNA 3WJ motif. We demonstrate that it can be stretched to wide conformations resulting in different 2D polygons: triangle (∠AOB = 60°) (18), square (∠AOB = 90°) and pentagon (∠AOB = 108°). Intermolecular interactions such as kissing loops, receptor loop, or ‘sticky-ends’ were avoided by introducing linkages through base pairing between corners of the polygons using RNA double helices. Therefore, this system is advantageous with an increased thermo-stability in the overall construct.
Affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Markey Cancer Center, Nanobiotechnology Center, University of Kentucky, Lexington, KY 40536, USA.